ArticleMolecular biology reports2013
Development of simple sequence repeat markers and diversity analysis in alfalfa (Medicago sativa L.).
Article in Molecular biology reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Simple Sequence Repeat-Based Genetic Diversity Analysis of Alfalfa Varieties.International journal of molecular sciences · 2025Article
- Characterization and Application of EST-SSR Markers Developed from Transcriptome Sequences inGenes · 2023Article
- Genome-wide development and application of miRNA-SSR markers inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Eco-Geographical, Morphological and Molecular Characterization of a Collection of the Perennial Endemic SpeciesPlants (Basel, Switzerland) · 2021Article
- Genetic diversity and population structure analysis in a large collection of white clover (PeerJ · 2021Article
- Development of transcriptome-wide SSR markers for genetic diversity and structure analysis inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Development of EST-SSR markers and association mapping with floral traits in Syringa oblata.BMC plant biology · 2020Article
- Genome-wide development of miRNA-based SSR markers in Cleistogenes songorica and analysis of their transferability to Gramineae/non-Gramineae species.Journal of applied genetics · 2020Article
- Development of polymorphic EST-SSR markers and their applicability in genetic diversity evaluation in Rhododendron arboreum.Molecular biology reports · 2020Article
- Comparative transcriptomic analysis reveals genetic divergence and domestication genes in Diospyros.BMC plant biology · 2019Article
- Development of novel EST-SSR markers in the macaúba palm (Acrocomia aculeata) using transcriptome sequencing and cross-species transferability in Arecaceae species.BMC plant biology · 2018Article
- Article
- Genetic variation and diversity in 199 Melilotus accessions based on a combination of 5 DNA sequences.PloS one · 2018Article
- Cross-species transferability of EST-SSR markers developed from the transcriptome of Melilotus and their application to population genetics research.Scientific reports · 2017Article
- Genome-Wide Development of MicroRNA-Based SSR Markers in Medicago truncatula with Their Transferability Analysis and Utilization in Related Legume Species.International journal of molecular sciences · 2017Article
- Comparative Transcriptome Analysis Reveals Adaptive Evolution of Notopterygium incisum and Notopterygium franchetii, Two High-Alpine Herbal Species Endemic to China.Molecules (Basel, Switzerland) · 2017Article
- Global transcriptome profiling analysis reveals insight into saliva-responsive genes in alfalfa.Plant cell reports · 2016Article
- Development and cross-species transferability of EST-SSR markers in Siberian wildrye (Elymus sibiricus L.) using Illumina sequencing.Scientific reports · 2016Article
- Development and Characterization of Transcription Factor Gene-Derived Microsatellite (TFGM) Markers in Medicago truncatula and Their Transferability in Leguminous and Non-Leguminous Species.Molecules (Basel, Switzerland) · 2015Article
- Analysis of BAC-end sequences in common bean (Phaseolus vulgaris L.) towards the development and characterization of long motifs SSRs.Plant molecular biology · 2014Article
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5 authors.
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Abstract
Efficient and robust molecular markers are essential for molecular breeding in plant. Compared to dominant and bi-allelic markers, multiple alleles of simple sequence repeat (SSR) markers are particularly informative and superior in genetic linkage map and QTL mapping in autotetraploid species like alfalfa. The objective of this study was to enrich SSR markers directly from alfalfa expressed sequence tags (ESTs). A total of 12,371 alfalfa ESTs were retrieved from the National Center for Biotechnology Information. Total 774 SSR-containing ESTs were identified from 716 ESTs. On average, one SSR was found per 7.7 kb of EST sequences. Tri-nucleotide repeats (48.8 %) was the most abundant motif type, followed by di-(26.1 %), tetra-(11.5 %), penta-(9.7 %), and hexanucleotide (3.9 %). One hundred EST-SSR primer pairs were successfully designed and 29 exhibited polymorphism among 28 alfalfa accessions. The allele number per marker ranged from two to 21 with an average of 6.8. The PIC values ranged from 0.195 to 0.896 with an average of 0.608, indicating a high level of polymorphism of the EST-SSR markers. Based on the 29 EST-SSR markers, assessment of genetic diversity was conducted and found that Medicago sativa ssp. sativa was clearly different from the other subspecies. The high transferability of those EST-SSR markers was also found for relative species.
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