ArticleSensors (Basel, Switzerland)2012
Repeatability, reproducibility and standardisation of a laser Doppler imaging technique for the evaluation of normal mouse hindlimb perfusion.
Article in Sensors (Basel, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Low-intensity ultrasound induces angiogenesis by activating endothelial integrin signaling in male mice.Physiological reports · 2026Article
- Microcirculatory impact of vatinoxan and fentanyl in male Wistar rats sedated with medetomidine and midazolam.BMC veterinary research · 2026Article
- Murine hindlimb ischemia models: a narrative review.Frontiers in cardiovascular medicine · 2025Review
- The quality and quantity media-cultured mononuclear cell transplantation is safe and effective in ischemic hindlimb mouse model.JVS-vascular science · 2023Article
- Simultaneous electroencephalography-functional magnetic resonance imaging for assessment of human brain function.Frontiers in systems neuroscience · 2022Review
- Normoxic low-altitude simulation (at 714 mmHg) improves limb blood perfusion in mice with hindlimb ischemia.Physiological reports · 2021Article
- Can Dynamic Contrast-Enhanced CT Quantify Perfusion in a Stimulated Muscle of Limited Size? A Rat Model.Clinical orthopaedics and related research · 2020Article
- Variations in Surgical Procedures for Inducing Hind Limb Ischemia in Mice and the Impact of These Variations on Neovascularization Assessment.International journal of molecular sciences · 2019Review
- Treating ischemia via recruitment of antigen-specific T cells.Science advances · 2019Article
- Simultaneous EEG-fMRI for Functional Neurological Assessment.Frontiers in neurology · 2019Review
- A systematic approach to assess locoregional differences in angiogenesis.Histochemistry and cell biology · 2016Article
- Effects of some anesthetic agents on skin microcirculation evaluated by laser Doppler perfusion imaging in mice.BMC veterinary research · 2013Article
- A Novel Method for Pulsometry Based on Traditional Iranian Medicine.Journal of medical signals and sensorsArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPreclinical perfusion studies are useful for the improvement of diagnosis and therapy in dermatologic, cardiovascular and rheumatic human diseases. The Laser Doppler Perfusion Imaging (LDPI) technique has been used to evaluate superficial alterations of the skin microcirculation in surgically induced murine hindlimb ischemia. We assessed the reproducibility and the accuracy of LDPI acquisitions and identified several critical factors that could affect LDPI measurements in mice.
methodsTwenty mice were analysed. Statistical standardisation and a repeatability and reproducibility analysis were performed on mouse perfusion signals with respect to differences in body temperature, the presence or absence of hair, the type of anaesthesia used for LDPI measurements and the position of the mouse body.
resultsWe found excellent correlations among measurements made by the same operator (i.e., repeatability) under the same experimental conditions and by two different operators (i.e., reproducibility). A Bland-Altman analysis showed the absence of bias in repeatability (p = 0.29) or reproducibility (p = 0.89). The limits of agreement for repeatability were -0.357 and -0.033, and for reproducibility, they were -0.270 and 0.238. Significant differences in perfusion values were observed in different experimental groups.
conclusionsDifferent experimental conditions must be considered as a starting point for the evaluation of new drugs and strategic therapies.
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