ArticleForensic science, medicine, and pathology2013
Successful direct amplification of nuclear markers from a single hair follicle.
Article in Forensic science, medicine, and pathology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed.
- Article
- Individual identification and kinship testing from hair shaft nuclear DNA: leveraging short amplicon strategy and bioinformatics models.International journal of legal medicine · 2026Article
- Detection of deletion/insertion polymorphism profiles from single human hair shafts.Molecular biology reports · 2022Article
- Direct PCR amplification from saliva sample using non-direct multiplex STR kits for forensic DNA typing.Scientific reports · 2021Article
- Article
- A proof of principal study on the use of direct PCR of semen and spermatozoa and development of a differential isolation protocol for use in cases of alleged sexual assault.International journal of legal medicine · 2017Article
- DNA profiles from clothing fibers using direct PCR.Forensic science, medicine, and pathology · 2016Article
- Direct-to-PCR tissue preservation for DNA profiling.International journal of legal medicine · 2016Article
- DNA profiles from fingernails using direct PCR.Forensic science, medicine, and pathology · 2015Article
- Forensic animal DNA analysis using economical two-step direct PCR.Forensic science, medicine, and pathology · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on successful amplification of DNA profiles from a single hair. Direct amplification was used on the root tip of both anagen and telogen hairs using a kit to amplify 15 STR loci. All 30 anagen hairs tested from five different people gave full DNA profiles after 29 cycles with no allelic drop-in or heterozygous imbalance. Six of the 30 telogen hairs tested resulted in a full DNA profile, and a further four telogen hair samples tested produced a DNA profile of five or more complete loci that could be up-loaded to the National DNA Database (Australia). A full DNA profile was also obtained from the shaft of an anagen hair. Current practice for many laboratories is that a single hair may not be subjected to DNA testing as there is little chance of success, hence this 100 % success rate from anagen hairs is a significant advancement. A full DNA profile was obtained from a 5 year-old single hair illustrating the success when using direct PCR rather than attempting an extraction prior to the amplification step. The process described deliberately uses current DNA profiling methods with no increase in cycle number, such that the methodology can be incorporated readily into operational practice. For the first time in the field of human identification, single hairs can be analyzed with confidence that a meaningful DNA profile will be generated and the data accepted by the criminal justice system.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.