ArticleAntimicrobial agents and chemotherapy2013
Mutational and transcriptomic changes involved in the development of macrolide resistance in Campylobacter jejuni.
Article in Antimicrobial agents and chemotherapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
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- Characterization of AreABC, an RND-Type Efflux System Involved in Antimicrobial Resistance of Aliarcobacter butzleri.Antimicrobial agents and chemotherapy · 2021Article
- High-throughput sequencing reveals genetic determinants associated with antibiotic resistance in Campylobacter spp. from farm-to-fork.PloS one · 2021Article
- Antimicrobial Resistance Determinants Circulating among ThermophilicAntibiotics (Basel, Switzerland) · 2020Article
- Prevalence, Population Diversity and Antimicrobial Resistance ofMicroorganisms · 2020Article
- Antibiotic Resistance Profiles and Molecular Mechanisms ofFrontiers in microbiology · 2020Article
- Validating the AMRFinder Tool and Resistance Gene Database by Using Antimicrobial Resistance Genotype-Phenotype Correlations in a Collection of Isolates.Antimicrobial agents and chemotherapy · 2019Article
- Twenty-seven-nucleotide repeat insertion in theOncotarget · 2018Article
- Novel mechanism responsible for high-level macrolide resistance inInfection and drug resistance · 2018Article
- Molecular Basis of Macrolide Resistance inBioMed research international · 2018Article
- Human Campylobacteriosis in Italy: Emergence of Multi-Drug Resistance to Ciprofloxacin, Tetracycline, and Erythromycin.Frontiers in microbiology · 2018Article
- The Current State of Macrolide Resistance in Campylobacter spp.: Trends and Impacts of Resistance Mechanisms.Applied and environmental microbiology · 2017Review
- Selection of Resistance to Clarithromycin in Mycobacterium abscessus Subspecies.Antimicrobial agents and chemotherapy · 2017Article
- Article
- Genotypic diversity, antimicrobial resistance and biofilm-forming abilities ofGut pathogens · 2017Article
- Article
- Whole-Genome Sequencing Analysis Accurately Predicts Antimicrobial Resistance Phenotypes in Campylobacter spp.Applied and environmental microbiology · 2016Article
- The Risk of Some Veterinary Antimicrobial Agents on Public Health Associated with Antimicrobial Resistance and their Molecular Basis.Frontiers in microbiology · 2016Review
- Virulence and Genomic Feature of Multidrug ResistantFrontiers in microbiology · 2016Article
- The Epidemiologic and Pharmacodynamic Cutoff Values of Tilmicosin against Haemophilus parasuis.Frontiers in microbiology · 2016Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Macrolide antibiotics are important for clinical treatment of infections caused by Campylobacter jejuni. Development of resistance to this class of antibiotics in Campylobacter is a complex process, and the dynamic molecular changes involved in this process remain poorly defined. Multiple lineages of macrolide-resistant mutants were selected by stepwise exposure of C. jejuni to escalating doses of erythromycin or tylosin. Mutations in target genes were determined by DNA sequencing, and the dynamic changes in the expression of antibiotic efflux transporters and the transcriptome of C. jejuni were examined by real-time reverse transcription-PCR, immunoblotting, and DNA microarray analysis. Multiple types of mutations in ribosomal proteins L4 and L22 occurred early during stepwise selection. On the contrary, the mutations in the 23S rRNA gene, mediating high resistance to macrolides, were observed only in the late-stage mutants. Upregulation of antibiotic efflux genes was observed in the intermediately resistant mutants, and the magnitude of upregulation declined with the occurrence of mutations in the 23S rRNA gene. DNA microarray analysis revealed the differential expression of 265 genes, most of which occurred in the intermediate mutant, including the upregulation of genes encoding ribosomal proteins and the downregulation of genes involved in energy metabolism and motility. These results indicate (i) that mutations in L4 and L22 along with temporal overexpression of antibiotic efflux genes precede and may facilitate the development of high-level macrolide resistance and (ii) that the development of macrolide resistance affects the pathways important for physiology and metabolism in C. jejuni, providing an explanation for the reduced fitness of macrolide-resistant Campylobacter.
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