ArticleStructure (London, England : 1993)2013
Structure of the essential diversity-generating retroelement protein bAvd and its functionally important interaction with reverse transcriptase.
Article in Structure (London, England : 1993), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Single cell viral tagging ofGut microbes · 2025Article
- Targeted protein evolution in the gut microbiome by diversity-generating retroelements.Science (New York, N.Y.) · 2025Article
- Article
- Targeted protein evolution in the gut microbiome by diversity-generating retroelements.bioRxiv : the preprint server for biology · 2024Article
- Genomic characterization of prophage elements inMicrobiology (Reading, England) · 2024Article
- Tentaclins-A Novel Family of Phage Receptor-Binding Proteins That Can Be Hypermutated by DGR Systems.International journal of molecular sciences · 2023Article
- Review
- Selective Isolation ofMicroorganisms · 2022Article
- Characterization and genomic analysis of a novel halovirus infectingFrontiers in microbiology · 2022Article
- Prokaryotic reverse transcriptases: from retroelements to specialized defense systems.FEMS microbiology reviews · 2021Review
- Article
- Determinants of adenine-mutagenesis in diversity-generating retroelements.Nucleic acids research · 2021Article
- Article
- Discovery and characterization of the evolution, variation and functions of diversity-generating retroelements using thousands of genomes and metagenomes.BMC genomics · 2019Article
- MyDGR: a server for identification and characterization of diversity-generating retroelements.Nucleic acids research · 2019Article
- Template-assisted synthesis of adenine-mutagenized cDNA by a retroelement protein complex.Nucleic acids research · 2018Article
- Diversity-generating retroelements: natural variation, classification and evolution inferred from a large-scale genomic survey.Nucleic acids research · 2018Article
- DGR mutagenic transposition occurs via hypermutagenic reverse transcription primed by nicked template RNA.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Conservation of the C-type lectin fold for accommodating massive sequence variation in archaeal diversity-generating retroelements.BMC structural biology · 2016Article
- Formylglycine, a post-translationally generated residue with unique catalytic capabilities and biotechnology applications.ACS chemical biology · 2015Review
Corrections and comments
- Commented on by
Authors and funding
8 authors.
Funding
Abstract
Diversity-generating retroelements (DGRs) are the only known source of massive protein sequence variation in prokaryotes. These elements transfer coding information from a template region (TR) through an RNA intermediate to a protein-encoding variable region. This retrohoming process is accompanied by unique adenine-specific mutagenesis and, in the prototypical BPP-1 DGR, requires a reverse transcriptase (bRT) and an accessory variability determinant (bAvd) protein. To understand the role of bAvd, we determined its 2.69 Å resolution structure, which revealed a highly positively charged pentameric barrel. In accordance with its charge, bAvd bound both DNA and RNA, albeit without a discernable sequence preference. We found that the coding sequence of bAvd functioned as part of TR but identified means to mutate bAvd without affecting TR. This mutational analysis revealed a strict correspondence between retrohoming and interaction of bAvd with bRT, suggesting that the bRT-bAvd complex is important for DGR retrohoming.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.