ArticleStructure (London, England : 1993)2013
Accurate high-throughput structure mapping and prediction with transition metal ion FRET.
Article in Structure (London, England : 1993), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- A PIP2-stabilized syntaxin-1a structure mapped with transition metal ion FRET and unnatural fluorescent amino acids at the plasma membrane.Structure (London, England : 1993) · 2026Article
- Structure-Based Understanding of CuBiosensors · 2025Review
- Deciphering DOM-metal binding using EEM-PARAFAC: Mechanisms, challenges, and perspectives.Environmental science and pollution research international · 2024Review
- Ultrafast Bioorthogonal Spin-Labeling and Distance Measurements in Mammalian Cells Using Small, Genetically Encoded Tetrazine Amino Acids.Journal of the American Chemical Society · 2023Article
- Ultra-Fast Bioorthogonal Spin-Labeling and Distance Measurements in Mammalian Cells Using Small, Genetically Encoded Tetrazine Amino Acids.bioRxiv : the preprint server for biology · 2023Article
- Article
- Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins.Frontiers in molecular biosciences · 2019Review
- Article
- Transition metal ion FRET uncovers KNature communications · 2016Article
- Regulation of CNGA1 Channel Gating by Interactions with the Membrane.The Journal of biological chemistry · 2016Article
- MamO Is a Repurposed Serine Protease that Promotes Magnetite Biomineralization through Direct Transition Metal Binding in Magnetotactic Bacteria.PLoS biology · 2016Article
- Transition metal ion FRET to measure short-range distances at the intracellular surface of the plasma membrane.The Journal of general physiology · 2016Article
- Measuring distances between TRPV1 and the plasma membrane using a noncanonical amino acid and transition metal ion FRET.The Journal of general physiology · 2016Article
- Article
- Targeted conformational search with map-restrained self-guided Langevin dynamics: application to flexible fitting into electron microscopic density maps.Journal of structural biology · 2013Article
- A secondary structural transition in the C-helix promotes gating of cyclic nucleotide-regulated ion channels.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Mapping the landscape of a protein's conformational space is essential to understanding its functions and regulation. The limitations of many structural methods have made this process challenging for most proteins. Here, we report that transition metal ion FRET (tmFRET) can be used in a rapid, highly parallel screen, to determine distances from multiple locations within a protein at extremely low concentrations. The distances generated through this screen for the protein maltose binding protein (MBP) match distances from the crystal structure to within a few angstroms. Furthermore, energy transfer accurately detects structural changes during ligand binding. Finally, fluorescence-derived distances can be used to guide molecular simulations to find low energy states. Our results open the door to rapid, accurate mapping and prediction of protein structures at low concentrations, in large complex systems, and in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.