ArticlePloS one2012
Nitric oxide participates in cold-inhibited Camellia sinensis pollen germination and tube growth partly via cGMP in vitro.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- Low-temperature stress modulates pollen tube growth through temperature-dependent multi-level regulatory mechanisms in Camellia sinensis.Plant reproduction · 2026Article
- Integration of NO and HPlant communications · 2026Review
- Nitric oxide in plants: an insight on redox activity and responses toward abiotic stress signaling.Plant signaling & behavior · 2024Review
- Negative Impacts of Arsenic on Plants and Mitigation Strategies.Plants (Basel, Switzerland) · 2023Review
- Nitric Oxide Metabolism Affects Germination inJournal of fungi (Basel, Switzerland) · 2022Article
- Let's shape again: the concerted molecular action that builds the pollen tube.Plant reproduction · 2022Review
- MicroRNA Omics Analysis ofBiomolecules · 2021Article
- Computational Identification of Functional Centers in Complex Proteins: A Step-by-Step Guide With Examples.Frontiers in bioinformatics · 2021Article
- Comparative transcriptomic analysis of the tea plant (Camellia sinensis) reveals key genes involved in pistil deletion.Hereditas · 2020Article
- Article
- Exogenous Melatonin Alleviates Cold Stress by Promoting Antioxidant Defense and Redox Homeostasis in Camellia sinensis L.Molecules (Basel, Switzerland) · 2018Article
- Discovery of Novel Functional Centers With Rationally Designed Amino Acid Motifs.Computational and structural biotechnology journal · 2018Review
- Ethylene promotes pollen tube growth by affecting actin filament organization via the cGMP-dependent pathway in Arabidopsis thaliana.Protoplasma · 2018Article
- Gene expression profile indicates involvement of NO in Camellia sinensis pollen tube growth at low temperature.BMC genomics · 2016Article
- Transcriptome analysis reveals self-incompatibility in the tea plant (Camellia sinensis) might be under gametophytic control.BMC genomics · 2016Article
- Combined Cytological and Transcriptomic Analysis Reveals a Nitric Oxide Signaling Pathway Involved in Cold-Inhibited Camellia sinensis Pollen Tube Growth.Frontiers in plant science · 2016Article
- Overexpression of Camellia sinensis H1 histone gene confers abiotic stress tolerance in transgenic tobacco.Plant cell reports · 2014Article
- RuBisCO depletion improved proteome coverage of cold responsive S-nitrosylated targets in Brassica juncea.Frontiers in plant science · 2013Article
- Gene regulation of iron-deficiency responses is associated with carbon monoxide and heme oxydase 1 in Chlamydomonas reinhardtii.PloS one · 2013Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitric oxide (NO) plays essential roles in many biotic and abiotic stresses in plant development procedures, including pollen tube growth. Here, effects of NO on cold stress inhibited pollen germination and tube growth in Camellia sinensis were investigated in vitro. The NO production, NO synthase (NOS)-like activity, cGMP content and proline (Pro) accumulation upon treatment with NO scavenger cPTIO, NOS inhibitor L-NNA, NO donor DEA NONOate, guanylate cyclase (GC) inhibitor ODQ or phosphodiesterase (PDE) inhibitor Viagra at 25°C (control) or 4°C were analyzed. Exposure to 4°C for 2 h reduced pollen germination and tube growth along with increase of NOS-like activity, NO production and cGMP content in pollen tubes. DEA NONOate treatment inhibited pollen germination and tube growth in a dose-dependent manner under control and reinforced the inhibition under cold stress, during which NO production and cGMP content promoted in pollen tubes. L-NNA and cPTIO markedly reduced the generation of NO induced by cold or NO donor along with partly reverse of cold- or NO donor-inhibited pollen germination and tube growth. Furthermore, ODQ reduced the cGMP content under cold stress and NO donor treatment in pollen tubes. Meanwhile, ODQ disrupted the reinforcement of NO donor on the inhibition of pollen germination and tube growth under cold condition. Additionally, Pro accumulation of pollen tubes was reduced by ODQ compared with that receiving NO donor under cold or control condition. Effects of cPTIO and L-NNA in improving cold-treated pollen germination and pollen tube growth could be lowered by Viagra. Moreover, the inhibitory effects of cPTIO and L-NNA on Pro accumulation were partly reversed by Viagra. These data suggest that NO production from NOS-like enzyme reaction decreased the cold-responsive pollen germination, inhibited tube growth and reduced Pro accumulation, partly via cGMP signaling pathway in C. sinensis.
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