ArticlePloS one2012
A combined approach for the assessment of cell viability and cell functionality of human fibrochondrocytes for use in tissue engineering.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 20 citations in OpenAlex.
- Basic Quality Controls Used in Skin Tissue Engineering.Life (Basel, Switzerland) · 2021Review
- Successful development and clinical translation of a novel anterior lamellar artificial cornea.Journal of tissue engineering and regenerative medicine · 2019Article
- Determination of the acute toxic effects of zinc oxide nanoparticles (ZnO NPs) in total hemocytes counts of Galleria mellonella (Lepidoptera: Pyralidae) with two different methods.Ecotoxicology (London, England) · 2019Article
- A steroidal derivative from Trigonella foenum graecum L. that induces apoptosis in vitro and in vivo.Journal of food and drug analysis · 2019Article
- Wharton's jelly-derived mesenchymal cells as a new source for the generation of microtissues for tissue engineering applications.Histochemistry and cell biology · 2018Article
- Encapsulation of human elastic cartilage-derived chondrocytes in nanostructured fibrin-agarose hydrogels.Histochemistry and cell biology · 2017Article
- Extrinsic and Intrinsic Mechanisms by Which Mesenchymal Stem Cells Suppress the Immune System.The ocular surface · 2016Review
- Immortalization and characterization of mouse temporomandibular joint disc cell clones with capacity for multi-lineage differentiation.Osteoarthritis and cartilage · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Temporo-mandibular joint disc disorders are highly prevalent in adult populations. Autologous chondrocyte implantation is a well-established method for the treatment of several chondral defects. However, very few studies have been carried out using human fibrous chondrocytes from the temporo-mandibular joint (TMJ). One of the main drawbacks associated to chondrocyte cell culture is the possibility that chondrocyte cells kept in culture tend to de-differentiate and to lose cell viability under in in-vitro conditions. In this work, we have isolated human temporo-mandibular joint fibrochondrocytes (TMJF) from human disc and we have used a highly-sensitive technique to determine cell viability, cell proliferation and gene expression of nine consecutive cell passages to determine the most appropriate cell passage for use in tissue engineering and future clinical use. Our results revealed that the most potentially viable and functional cell passages were P5-P6, in which an adequate equilibrium between cell viability and the capability to synthesize all major extracellular matrix components exists. The combined action of pro-apoptotic (TRAF5, PHLDA1) and anti-apoptotic genes (SON, HTT, FAIM2) may explain the differential cell viability levels that we found in this study. These results suggest that TMJF should be used at P5-P6 for cell therapy protocols.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.