ArticlePloS one2012
Evidence for small RNAs homologous to effector-encoding genes and transposable elements in the oomycete Phytophthora infestans.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 88 citations in OpenAlex.
- Taxonomy, diversity, and effects of viruses infecting Phytophthora spp.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Review
- Effector gene silencing coordinated by histone methylation and small RNAs enhances host adaptation in a plant pathogen.Nucleic acids research · 2026Article
- Phytophthora capsici carries and differentially expresses genes for the RNA interference pathway.PloS one · 2026Article
- Pathogenicity and virulence ofVirulence · 2025Review
- RNA Silencing Strategies in Phytophthora: Experimental Guidelines and Insights.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Adaptive regulation of miRNAs/milRNAs in tissue-specific interaction between apple andHorticulture research · 2024Article
- Distinct small RNAs are expressed at different stages ofFrontiers in genetics · 2024Article
- RNA silencing proteins and small RNAs in oomycete plant pathogens and biocontrol agents.Frontiers in microbiology · 2023Article
- Article
- Mutations in PpAGO3 Lead to Enhanced Virulence ofFrontiers in microbiology · 2022Article
- smartPARE: An R Package for Efficient Identification of True mRNA Cleavage Sites.International journal of molecular sciences · 2021Article
- Multiple Viral Infections Detected inViruses · 2021Article
- Characterization ofFrontiers in microbiology · 2021Article
- Diverse Trajectories Drive the Expression of a Giant Virus in the Oomycete Plant PathogenFrontiers in microbiology · 2021Article
- The Evolutionary Significance of RNAi in the Fungal Kingdom.International journal of molecular sciences · 2020Review
- Article
- Rapid adaptation of the Irish potato famine pathogenEvolutionary applications · 2020Article
- Effector gene silencing mediated by histone methylation underpins host adaptation in an oomycete plant pathogen.Nucleic acids research · 2020Article
- Characterization of Two Satellite DNA Families in the Genome of the Oomycete Plant PathogenFrontiers in genetics · 2020Article
- Dominance of Mating Type A1 and Indication of Epigenetic Effects During Early Stages of Mating inFrontiers in microbiology · 2020Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Phytophthora infestans is the oomycete pathogen responsible for the devastating late blight disease on potato and tomato. There is presently an intense research focus on the role(s) of effectors in promoting late blight disease development. However, little is known about how they are regulated, or how diversity in their expression may be generated among different isolates. Here we present data from investigation of RNA silencing processes, characterized by non-coding small RNA molecules (sRNA) of 19-40 nt. From deep sequencing of sRNAs we have identified sRNAs matching numerous RxLR and Crinkler (CRN) effector protein genes in two isolates differing in pathogenicity. Effector gene-derived sRNAs were present in both isolates, but exhibited marked differences in abundance, especially for CRN effectors. Small RNAs in P. infestans grouped into three clear size classes of 21, 25/26 and 32 nt. Small RNAs from all size classes mapped to RxLR effector genes, but notably 21 nt sRNAs were the predominant size class mapping to CRN effector genes. Some effector genes, such as PiAvr3a, to which sRNAs were found, also exhibited differences in transcript accumulation between the two isolates. The P. infestans genome is rich in transposable elements, and the majority of sRNAs of all size classes mapped to these sequences, predominantly to long terminal repeat (LTR) retrotransposons. RNA silencing of Dicer and Argonaute genes provided evidence that generation of 21 nt sRNAs is Dicer-dependent, while accumulation of longer sRNAs was impacted by silencing of Argonaute genes. Additionally, we identified six microRNA (miRNA) candidates from our sequencing data, their precursor sequences from the genome sequence, and target mRNAs. These miRNA candidates have features characteristic of both plant and metazoan miRNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.