ArticlePloS one2012
Spastin's microtubule-binding properties and comparison to katanin.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 64 citations in OpenAlex.
- Microtubule lattice defects facilitate spastin-mediated severing.Journal of cell science · 2026Article
- Surviving desiccation: key factors underlying tolerance in prokaryotes and eukaryotes.Protoplasma · 2025Review
- Elucidating the Functional Roles of Long Non-Coding RNAs in Alzheimer's Disease.International journal of molecular sciences · 2024Article
- Herding of proteins by the ends of shrinking polymers.Physical review. E · 2023Article
- A Tale of 12 Tails: Katanin Severing Activity Affected by Carboxy-Terminal Tail Sequences.Biomolecules · 2023Article
- Article
- Precise control of microtubule disassembly in living cells.The EMBO journal · 2022Article
- Spastin interacts with collapsin response mediator protein 3 to regulate neurite growth and branching.Neural regeneration research · 2021Article
- Modeling a disease-correlated tubulin mutation in budding yeast reveals insight into MAP-mediated dynein function.Molecular biology of the cell · 2021Article
- WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin.The Journal of cell biology · 2021Article
- Cutting, Amplifying, and Aligning Microtubules with Severing Enzymes.Trends in cell biology · 2021Review
- Kinetic Analysis of AAA+ Translocases by Combined Fluorescence and Anisotropy Methods.Biophysical journal · 2020Article
- A novel SPAST gene mutation identified in a Chinese family with hereditary spastic paraplegia.BMC medical genetics · 2020Article
- A nuclear localization signal is required for the nuclear translocation of Fign and its microtubule‑severing function.Molecular medicine reports · 2020Article
- Katanin Grips the β-Tubulin Tail through an Electropositive Double Spiral to Sever Microtubules.Developmental cell · 2020Article
- Article
- An allosteric network in spastin couples multiple activities required for microtubule severing.Nature structural & molecular biology · 2019Article
- Spastin is a dual-function enzyme that severs microtubules and promotes their regrowth to increase the number and mass of microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Katanin catalyzes microtubule depolymerization independently of tubulin C-terminal tails.Cytoskeleton (Hoboken, N.J.) · 2019Article
- Katanin-like protein Katnal2 is required for ciliogenesis and brain development in Xenopus embryos.Developmental biology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spastin and katanin are ring-shaped hexameric AAA ATPases that sever microtubules, and thus crucially depend on a physical interaction with microtubules. For the first time, we report here the microtubule binding properties of spastin at the single-molecule level, and compare them to katanin. Microscopic fluorescence assays showed that human spastin bound to microtubules by ionic interactions, and diffused along microtubules with a diffusion coefficient comparable to katanin. The microscopic measurement of landing and dissociation rates demonstrated the ionic character of the interaction, which could be mapped to a patch of three lysine residues outside of the catalytic domain of human spastin. This motif is not conserved in Drosophila spastin or katanin, which also bound by non-catalytic parts of the protein. The binding affinities of spastin and katanin were nucleotide-sensitive, with the lowest affinities under ADP,, the highest under ATP-γS conditions. These changes correlated with the formation of higher oligomeric states, as shown in biochemical experiments and electron microscopic images. Vice versa, the artificial dimerization of human spastin by addition of a coiled coil led to a constitutively active enzyme. These observations suggest that dimer formation is a crucial step in the formation of the active complex, and thus the severing process by spastin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.