ArticleGenetics and molecular biology2012
Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum.
Article in Genetics and molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Spotting the Targets of the Apospory ControllerPlants (Basel, Switzerland) · 2022Article
- The Auxin-Response RepressorPlants (Basel, Switzerland) · 2022Article
- Differential Epigenetic Marks Are Associated with Apospory Expressivity in Diploid Hybrids ofPlants (Basel, Switzerland) · 2021Article
- A study of the heterochronic sense/antisense RNA representation in florets of sexual and apomictic Paspalum notatum.BMC genomics · 2021Article
- Review
- Coexpression and Transcriptome analyses identify active Apomixis-related genes in Paspalum notatum leaves.BMC genomics · 2020Article
- Retro-ElementFrontiers in genetics · 2020Article
- Small RNA-seq reveals novel regulatory components for apomixis in Paspalum notatum.BMC genomics · 2019Article
- Heterochronic reproductive developmental processes between diploid and tetraploid cytotypes of Paspalum rufum.Annals of botany · 2019Article
- A Plant-SpecificFrontiers in plant science · 2019Article
- The vesicle trafficking regulator PN_SCD1 is demethylated and overexpressed in florets of apomictic Paspalum notatum genotypes.Scientific reports · 2018Article
- A reference floral transcriptome of sexual and apomictic Paspalum notatum.BMC genomics · 2017Article
- Identification of protease m1 zinc metalloprotease conferring resistance to deltamethrin by characterization of an AFLP marker in Culex pipiens pallens.Parasites & vectors · 2016Article
- Article
- PnTgs1-like expression during reproductive development supports a role for RNA methyltransferases in the aposporous pathway.BMC plant biology · 2014Article
- Harnessing apomictic reproduction in grasses: what we have learned from Paspalum.Annals of botany · 2013Review
- Apomixis in plant reproduction: a novel perspective on an old dilemma.Plant reproduction · 2013Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum. The objective of this work was to sequence these markers, obtain their flanking regions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen molecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence mapping in silico in the apospory-syntenic regions of rice and maize. Approximately 5 kb corresponding to different markers were characterized through the global sequencing procedure. A more refined analysis based on sequence information indicated synteny with segments of chromosomes 2 and 12 of rice and chromosomes 3 and 5 of maize. Two loci associated with apomixis locus were tested in methylation-sensitive RFLP experiments using genomic DNA extracted from leaves. Although both target sequences were methylated no methylation polymorphisms associated with the mode of reproduction were detected.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.