ArticleProceedings of the National Academy of Sciences of the United States of America2013
Tangled bank of experimentally evolved Burkholderia biofilms reflects selection during chronic infections.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 129 papers.
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Who cites it
129 citing papers in PubMed, 204 citations in OpenAlex.
- Trial
- Simulating the host niche: balancing complexity and control in the experimental evolution of antibiotic resistance and pathoadaptation.Microbiology (Reading, England) · 2026Review
- Disruption of bacteriophage integration site promotes rapid diversification of multicellular traits inMicrobiology spectrum · 2026Article
- Genetic diversification ofApplied and environmental microbiology · 2026Article
- Rapid evolution of Klebsiella pneumoniae biofilms in vitro delineates adaptive changes selected during infection.Nature communications · 2026Article
- Putting BASIL in a BLT: A Bayesian filtering method for estimating the fitness effects of nascent adaptive mutations.PLoS computational biology · 2026Article
- Exploring cooperation among social pathogens: a computational perspective.FEMS microbiology reviews · 2026Review
- Mixed-species interactions constrain diversification and shape biofilm evolution.ISME communications · 2026Article
- Competition between Pseudomonas species constrains ecological diversification in polymicrobial biofilms.NPJ biofilms and microbiomes · 2025Article
- Parallel within-host evolution alters virulence factors in an opportunistic Klebsiella pneumoniae during a hospital outbreak.Nature communications · 2025Article
- Ecological diversification in rapidly evolving populations.bioRxiv : the preprint server for biology · 2025Article
- Evolution of genotypic and phenotypic diversity in multispecies biofilms.NPJ biofilms and microbiomes · 2025Article
- Student-led experimental evolution reveals novel biofilm regulators of adaptation to multiple niches.bioRxiv : the preprint server for biology · 2025Article
- Co-Existence Slows Diversification in Experimental Populations of E. coli and P. fluorescens.Environmental microbiology · 2025Article
- Hypermutability bypasses genetic constraints in SCV phenotypic switching in Pseudomonas aeruginosa biofilms.NPJ biofilms and microbiomes · 2025Article
- Synergistic biodegradation of polyethylene by experimentally evolved bacterial biofilms.The ISME journal · 2025Article
- Presence of a biofilm beneficiary alters the evolutionary trajectory of a biofilm former.The ISME journal · 2025Article
- Antisense phosphorodiamidate morpholino oligomers retain activity inFrontiers in microbiology · 2025Article
- Experimental evolution reveals evolutionary bias and its causes.BMC ecology and evolution · 2024Article
- Microbial diversification is maintained in an experimentally evolved synthetic community.mSystems · 2024Article
69 more citing papers are in PubMed but not listed here.
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4 authors at 1 institution in 1 country.
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Abstract
How diversity evolves and persists in biofilms is essential for understanding much of microbial life, including the uncertain dynamics of chronic infections. We developed a biofilm model enabling long-term selection for daily adherence to and dispersal from a plastic bead in a test tube. Focusing on a pathogen of the cystic fibrosis lung, Burkholderia cenocepacia, we sequenced clones and metagenomes to unravel the mutations and evolutionary forces responsible for adaptation and diversification of a single biofilm community during 1,050 generations of selection. The mutational patterns revealed recurrent evolution of biofilm specialists from generalist types and multiple adaptive alleles at relatively few loci. Fitness assays also demonstrated strong interference competition among contending mutants that preserved genetic diversity. Metagenomes from five other independently evolved biofilm lineages revealed extraordinary mutational parallelism that outlined common routes of adaptation, a subset of which was found, surprisingly, in a planktonic population. These mutations in turn were surprisingly well represented among mutations that evolved in cystic fibrosis isolates of both Burkholderia and Pseudomonas. These convergent pathways included altered metabolism of cyclic diguanosine monophosphate, polysaccharide production, tricarboxylic acid cycle enzymes, global transcription, and iron scavenging. Evolution in chronic infections therefore may be driven by mutations in relatively few pathways also favored during laboratory selection, creating hope that experimental evolution may illuminate the ecology and selective dynamics of chronic infections and improve treatment strategies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.