ArticleThe Journal of biological chemistry2013
Subcomplexes of ancestral respiratory complex I subunits rapidly turn over in vivo as productive assembly intermediates in Arabidopsis.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Plant supercomplex I + III2 structure and function: implications for the growing field.Biochemical Society transactions · 2024Review
- Lessons from the deep: mechanisms behind diversification of eukaryotic protein complexes.Biological reviews of the Cambridge Philosophical Society · 2023Article
- The biogenesis and regulation of the plant oxidative phosphorylation system.Plant physiology · 2023Review
- The mitochondrial AAA protease FTSH3 regulates Complex I abundance by promoting its disassembly.Plant physiology · 2021Article
- Mitochondrial CLPP2 Assists Coordination and Homeostasis of Respiratory Complexes.Plant physiology · 2020Article
- The composition and turnover of the Arabidopsis thaliana 80S cytosolic ribosome.The Biochemical journal · 2020Article
- A Mitochondrial LYR Protein Is Required for Complex I Assembly.Plant physiology · 2019Article
- Co-ordination of NDH and Cup proteins in CO2 uptake in cyanobacterium Synechocystis sp. PCC 6803.Journal of experimental botany · 2017Article
- Mitochondrial Lon1 has a role in homeostasis of the mitochondrial ribosome and pentatricopeptide repeat proteins in plants.Plant signaling & behavior · 2017Article
- Life without complex I: proteome analyses of an Arabidopsis mutant lacking the mitochondrial NADH dehydrogenase complex.Journal of experimental botany · 2016Article
- The CA domain of the respiratory complex I is required for normal embryogenesis in Arabidopsis thaliana.Journal of experimental botany · 2016Article
- Identification of cleavage sites and substrate proteins for two mitochondrial intermediate peptidases in Arabidopsis thaliana.Journal of experimental botany · 2015Article
- Proteins with high turnover rate in barley leaves estimated by proteome analysis combined with in planta isotope labeling.Plant physiology · 2014Article
- An Arabidopsis stomatin-like protein affects mitochondrial respiratory supercomplex organization.Plant physiology · 2014Article
- The evolutionarily conserved iron-sulfur protein INDH is required for complex I assembly and mitochondrial translation in Arabidopsis [corrected].The Plant cell · 2013Article
- 3D Gel Map of Arabidopsis Complex I.Frontiers in plant science · 2013Article
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8 authors.
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Abstract
Subcomplexes of mitochondrial respiratory complex I (CI; EC 1.6.5.3) are shown to turn over in vivo, and we propose a role in an ancestral assembly pathway. By progressively labeling Arabidopsis cell cultures with (15)N and isolating mitochondria, we have identified CI subcomplexes through differences in (15)N incorporation into their protein subunits. The 200-kDa subcomplex, containing the ancestral γ-carbonic anhydrase (γ-CA), γ-carbonic anhydrase-like, and 20.9-kDa subunits, had a significantly higher turnover rate than intact CI or CI+CIII(2). In vitro import of precursors for these CI subunits demonstrated rapid generation of subcomplexes and revealed that their specific abundance varied when different ancestral subunits were imported. Time course studies of precursor import showed the further assembly of these subcomplexes into CI and CI+CIII(2), indicating that the subcomplexes are productive intermediates of assembly. The strong transient incorporation of new subunits into the 200-kDa subcomplex in a γ-CA mutant is consistent with this subcomplex being a key initiator of CI assembly in plants. This evidence alongside the pattern of coincident occurrence of genes encoding these particular proteins broadly in eukaryotes, except for opisthokonts, provides a framework for the evolutionary conservation of these accessory subunits and evidence of their function in ancestral CI assembly.
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