ArticleMycorrhiza2013
Tuber aestivum Vittad. mycelium quantified: advantages and limitations of a qPCR approach.
Article in Mycorrhiza, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- Rapid detection of cultivated truffle fungi (Applied and environmental microbiology · 2026Article
- Soil disturbance influences ectomycorrhizal fungal communities: an in-situ experiment under Mediterranean holm oak (Quercus ilex L.).Mycorrhiza · 2026Article
- Protists are key players in the utilization of protein nitrogen in the arbuscular mycorrhizal hyphosphere.The New phytologist · 2025Article
- Newly Designed Fluorescence In Situ Hybridization Probes Reveal Previously Unknown Endophytic Abilities of Tuber magnatum in Herbaceous Plants.Microbial ecology · 2025Article
- Hidden fairy rings and males-Genetic patterns of natural Burgundy truffle (Tuber aestivum Vittad.) populations reveal new insights into its life cycle.Environmental microbiology · 2022Article
- Design and Validation of qPCR-Specific Primers for Quantification of the MarketedJournal of fungi (Basel, Switzerland) · 2022Article
- White mulch and irrigation increase black truffle soil mycelium when competing with summer truffle in young truffle orchards.Mycorrhiza · 2021Article
- Arbuscular mycorrhizal fungi favor invasive Echinops sphaerocephalus when grown in competition with native Inula conyzae.Scientific reports · 2020Article
- Biofilm and planktonic microbial communities in highly acidic soil (pH < 3) in the Soos National Nature Reserve, Czech Republic.Extremophiles : life under extreme conditions · 2020Article
- Food Authentication: Identification and Quantitation of Different Tuber Species via Capillary Gel Electrophoresis and Real-Time PCR.Foods (Basel, Switzerland) · 2020Article
- Soil temperature and hydric potential influences the monthly variations of soil Tuber aestivum DNA in a highly productive orchard.Scientific reports · 2019Article
- 16S rRNA Gene Sequencing Reveals a Shift in the Microbiota ofFrontiers in microbiology · 2019Article
- Utilization of organic nitrogen by arbuscular mycorrhizal fungi-is there a specific role for protists and ammonia oxidizers?Mycorrhiza · 2018Article
- Seasonal dynamics of extraradical mycelium and mycorrhizas in a black truffle (Tuber melanosporum) plantation.Mycorrhiza · 2017Article
- Truffle biogeography-A case study revealing ecological niche separation of differentEcology and evolution · 2017Article
- Soil drying procedure affects the DNA quantification of Lactarius vinosus but does not change the fungal community composition.Mycorrhiza · 2016Article
- Quantification of arbuscular mycorrhizal fungal DNA in roots: how important is material preservation?Mycorrhiza · 2015Article
- Tuber aestivum association with non-host roots.Mycorrhiza · 2014Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A quantitative real-time PCR (qPCR) marker Ta0 with hydrolysis probe ("TaqMan"), targeted to the internal transcribed spacer region of the ribosomal DNA, has been developed for quantification of summer truffle (Tuber aestivum) mycelium. Gene copy concentrations determined by the qPCR were calibrated against pure culture mycelium of T. aestivum, enabling quantification of the mycelium in soil and in host roots from the fields. Significant concentrations of the fungus were observed not only in the finest roots with ectomycorrhizae but also in other root types, indicating that the fungus is an important component of the microbial film at the root surface. The concentration of T. aestivum in soil is relatively high compared to other ectomycorrhizal fungi. To evaluate the reliability of the measurement of the soil mycelium density using qPCR, the steady basal extracellular concentration of the stabilized T. aestivum DNA should be known and taken into account. Therefore, we addressed the stability of the qPCR signal in soil subjected to different treatments. After the field soil was sieved, regardless of whether it was dried/rewetted or not, the T. aestivum DNA was quickly decomposed. It took just about 4 days to reach a steady concentration. This represents a conserved pool of T. aestivum DNA and determines detection limit of the qPCR quantification in our case. When the soil was autoclaved and recolonized by saprotrophic microorganisms, this conserved DNA pool was eliminated and the soil became free of T. aestivum DNA.
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