ArticleMolecules (Basel, Switzerland)2012
Proteomic and functional analyses reveal MAPK1 regulates milk protein synthesis.
Article in Molecules (Basel, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- The application of omics technologies for understanding tropical plants-based bioactive compounds in ruminants: a review.Journal of animal science and biotechnology · 2024Review
- Advancing breastfeeding promotion: leveraging integrative natural galactagogues and unveiling their potential roles-Insights from a 19-year Taiwan nationwide registry to address lactation insufficiency in postpartum women.Frontiers in nutrition · 2024Article
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- Differences in the microRNAs Levels of Raw Milk from Dairy Cattle Raised under Extensive or Intensive Production Systems.Veterinary sciences · 2022Article
- Review
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- Characterization of RNA Editome in the Mammary Gland of Yaks during the Lactation and Dry Periods.Animals : an open access journal from MDPI · 2022Article
- circ-016910 sponges miR-574-5p to regulate cell physiology and milk synthesis via MAPK and PI3K/AKT-mTOR pathways in GMECs.Journal of cellular physiology · 2020Article
- In-depth proteome analysis of more than 12,500 proteins in buffalo mammary epithelial cell line identifies protein signatures for active proliferation and lactation.Scientific reports · 2020Article
- Article
- Establishment of a 3D cell culture model of primary bovine mammary epithelial cells extracted from fresh milk.In vitro cellular & developmental biology. Animal · 2017Article
- The Use of "Omics" in Lactation Research in Dairy Cows.International journal of molecular sciences · 2017Review
- Regulation of peroxisome proliferator-activated receptor gamma on milk fat synthesis in dairy cow mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2016Article
- 14-3-3γ regulates cell viability and milk fat synthesis in lipopolysaccharide-induced dairy cow mammary epithelial cells.Experimental and therapeutic medicine · 2016Article
- 14-3-3γ affects mTOR pathway and regulates lactogenesis in dairy cow mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2015Article
- 14-3-3γ Regulates Lipopolysaccharide-Induced Inflammatory Responses and Lactation in Dairy Cow Mammary Epithelial Cells by Inhibiting NF-κB and MAPKs and Up-Regulating mTOR Signaling.International journal of molecular sciences · 2015Article
- Functional analysis of FABP3 in the milk fat synthesis signaling pathway of dairy cow mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2014Article
- Function of SREBP1 in the milk fat synthesis of dairy cow mammary epithelial cells.International journal of molecular sciences · 2014Article
- GSK3β regulates milk synthesis in and proliferation of dairy cow mammary epithelial cells via the mTOR/S6K1 signaling pathway.Molecules (Basel, Switzerland) · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
L-Lysine (L-Lys) is an essential amino acid that plays fundamental roles in protein synthesis. Many nuclear phosphorylated proteins such as Stat5 and mTOR regulate milk protein synthesis. However, the details of milk protein synthesis control at the transcript and translational levels are not well known. In this current study, a two-dimensional gel electrophoresis (2-DE)/MS-based proteomic technology was used to identify phosphoproteins responsible for milk protein synthesis in dairy cow mammary epithelial cells (DCMECs). The effect of L-Lys on DCMECs was analyzed by CASY technology and reversed phase high performance liquid chromatography (RP-HPLC). The results showed that cell proliferation ability and β-casein expression were enhanced in DCMECs treated with L-Lys. By phosphoproteomics analysis, six proteins, including MAPK1, were identified up-expressed in DCMECs treated with 1.2 mM L-Lys for 24 h, and were verified by quantitative real-time PCR (qRT-PCR) and western blot. Overexpression and siRNA inhibition of MAPK1 experiments showed that MAPK1 upregulated milk protein synthesis through Stat5 and mTOR pathway. These findings that MAPK1 involves in regulation of milk synthesis shed new insights for understanding the mechanisms of milk protein synthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.