ReviewPflugers Archiv : European journal of physiology2013
Frontiers of protein expression control with conditional degrons.
Review in Pflugers Archiv : European journal of physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- The auxin-inducible degradation system enables conditional PERIOD protein depletion in the nervous system of Drosophila melanogaster.The FEBS journal · 2018Article
- Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis.Journal of cell science · 2018Article
- Dual regulation of cytoplasmic and mitochondrial acetyl-CoA utilization for improved isoprene production in Saccharomyces cerevisiae.Nature communications · 2016Article
- Selectable one-step PCR-mediated integration of a degron for rapid depletion of endogenous human proteins.BioTechniques · 2016Article
- Remote and reversible inhibition of neurons and circuits by small molecule induced potassium channel stabilization.Scientific reports · 2016Article
- Regulated protein depletion by the auxin-inducible degradation system in Drosophila melanogaster.Fly · 2016Article
- Peptide aptamers define distinct EB1- and EB3-binding motifs and interfere with microtubule dynamics.Molecular biology of the cell · 2014Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is useful to artificially control the expression levels of a protein of interest (POI), not only for its characterization in vivo, but also for the modulation of biological pathways. Overexpression of a POI is relatively easy because it is possible to drive its expression from a transgene encoding the POI under the control of a strong promoter. However, it is more challenging to reduce or deplete the expression of a POI. A protein domain called "degron", which induces rapid proteolysis by the proteasome, can be used for this purpose. Degron-based technologies for the conditional depletion of POI--degron fusion proteins have been developed by exploiting various pathways leading to proteasomal degradation. Compared with other depletion technologies that control the expression levels of POIs at the DNA or mRNA levels, these protein-depletion approaches are advantageous in terms of specificity, reversibility, and the time required for depletion. Current conditional degron-based technologies are described and discussed.
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Registered trials
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