ArticleIn vitro cellular & developmental biology. Animal2013
Wolbachia from the planthopper Laodelphax striatellus establishes a robust, persistent, streptomycin-resistant infection in clonal mosquito cells.
Article in In vitro cellular & developmental biology. Animal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 34 citations in OpenAlex.
- Cycloheximide, a specific inhibitor of eukaryotic protein synthesis, enhances infection of mosquito cells with Wolbachia.In vitro cellular & developmental biology. Animal · 2026Article
- Resistance ofInsects · 2024Article
- Establishment and partial characterisation of a new cell line derived from adult tissues of the tsetse fly Glossina morsitans morsitans.Parasites & vectors · 2024Article
- Virus-like Particles fromInsects · 2023Article
- Mitotically inactivated mosquito cells support robust Wolbachia infection and replication.In vitro cellular & developmental biology. Animal · 2022Article
- From Mosquito Ovaries to Ecdysone; from Ecdysone toInsects · 2022Review
- Article
- Isolation in Natural Host Cell Lines ofInsects · 2021Article
- Growth and Maintenance ofInsects · 2021Review
- Assessment of mitotically inactivated mosquito cell feeder layers produced with mitomycin C.In vitro cellular & developmental biology. Animal · 2021Article
- DNA recombination and repair in Wolbachia: RecA and related proteins.Molecular genetics and genomics : MGG · 2021Article
- Isolation and Propagation of Laboratory Strains and a Novel Flea-Derived Field Strain ofMicroorganisms · 2020Article
- Viral RNA is a target for Wolbachia-mediated pathogen blocking.PLoS pathogens · 2020Article
- Conditions facilitating infection of mosquito cell lines with Wolbachia, an obligate intracellular bacterium.In vitro cellular & developmental biology. Animal · 2019Article
- Strain-specific response to ampicillin in Wolbachia-infected mosquito cell lines.In vitro cellular & developmental biology. Animal · 2018Article
- Article
- Proteomic analysis of a mosquito host cell response to persistent Wolbachia infection.Research in microbiology · 2017Article
- Variable Inhibition of Zika Virus Replication by Different Wolbachia Strains in Mosquito Cell Cultures.Journal of virology · 2017Article
- The Wolbachia WO bacteriophage proteome in the Aedes albopictus C/wStr1 cell line: evidence for lytic activity?In vitro cellular & developmental biology. Animal · 2016Article
- Mosaic composition of ribA and wspB genes flanking the virB8-D4 operon in the Wolbachia supergroup B-strain, wStr.Archives of microbiology · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The obligate intracellular bacterium, Wolbachia pipientis (Rickettsiales: Anaplasmataceae), distorts reproduction of its arthropod hosts to facilitate invasion of naïve populations. This property makes Wolbachia an attractive "gene drive" agent with potential applications in the control of insect vector populations. Genetic manipulation of Wolbachia will require in vitro systems for its propagation, genetic modification, amplification, and introduction into target insects. Here we show that Wolbachia from the planthopper, Laodelphax striatellus, establishes a robust infection in clonal C7-10 Aedes albopictus mosquito cells. Infected cells, designated C/wStr, expressed radiolabeled proteins that were enriched in cells grown in the absence of antibiotics that inhibit Wolbachia, relative to cultures grown in medium containing tetracycline and rifampicin. Using mass spectrometry, we verified that tryptic peptides from an upregulated 24 kDa band predominantly represented proteins encoded by the Wolbachia genome, including the outer surface protein, Wsp. We further showed that resistance of Wolbachia to streptomycin is associated with a K42R mutation in Wolbachia ribosomal protein S12, and that the pattern of amino acid substitutions in ribosomal protein S12 shows distinct differences in the closely related genera, Wolbachia and Rickettsia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.