ArticleJournal of veterinary science2012
Application of a multiplex PCR assay for Campylobacter fetus detection and subspecies differentiation in uncultured samples of aborted bovine fetuses.
Article in Journal of veterinary science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Bovine campylobacteriosis in heifer: pathogenesis study and insights in the conventional and molecular diagnosis in an experimental bovine model and field cases.Veterinary research communications · 2024Article
- Whole-genome comparison using complete genomes fromFrontiers in microbiology · 2024Article
- Accurate and fast identification ofVeterinary and animal science · 2021Article
- Article
- Evaluation of intracellular survival ofIranian journal of veterinary research · 2021Article
- Assessment of Campylobacter fetus subsp. venerealis molecular diagnosis using clinical samples of bulls.BMC veterinary research · 2020Article
- Phylogenomic Analysis ofFrontiers in microbiology · 2020Article
- Pathogenomics of EmergingClinical microbiology reviews · 2019Review
- Review of Diagnostic Procedures and Approaches to Infectious Causes of Reproductive Failures of Cattle in Australia and New Zealand.Frontiers in veterinary science · 2018Review
- Diagnosis of Bovine Genital Campylobacteriosis in South America.Frontiers in veterinary science · 2018Review
- Myositis Ossificans of the Hip Due to Pyogenic Arthritis Caused by Campylobacter fetus Subspecies fetus.Internal medicine (Tokyo, Japan) · 2017Article
- A novel real-time PCR assay for quantitative detection of Campylobacter fetus based on ribosomal sequences.BMC veterinary research · 2016Article
- A rural worker infected with a bovine-prevalent genotype of Campylobacter fetus subsp. fetus supports zoonotic transmission and inconsistency of MLST and whole-genome typing.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Inconsistency of phenotypic and genomic characteristics of Campylobacter fetus subspecies requires reevaluation of current diagnostics.Journal of clinical microbiology · 2014Article
- Campylobacter shared between free-ranging cattle and sympatric wild ungulates in a natural environment (NE Spain).EcoHealth · 2014Article
- Survey of infectious etiologies of bovine abortion during mid- to late gestation in dairy herds.PloS one · 2014Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Campylobacter (C.) fetus (epsilonproteobacteria) is an important veterinary pathogen. This species is currently divided into C. fetus subspecies (subsp.) fetus (Cff) and C. fetus subsp. venerealis (Cfv). Cfv is the causative agent of bovine genital Campylobacteriosis, an infectious disease that leads to severe reproductive problems in cattle worldwide. Cff is a more general pathogen that causes reproductive problems mainly in sheep although cattle can also be affected. Here we describe a multiplex PCR method to detect C. fetus and differentiate between subspecies in a single step. The assay was standardized using cultured strains and successfully used to analyze the abomasal liquid of aborted bovine fetuses without any pre-enrichment step. Results of our assay were completely consistent with those of traditional bacteriological diagnostic methods. Furthermore, the multiplex PCR technique we developed may be easily adopted by any molecular diagnostic laboratory as a complementary tool for detecting C. fetus subspecies and obtaining epidemiological information about abortion events in cattle.
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Registered trials
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