ArticleMolecular biology reports2013
Application of qRT-PCR and RNA-Seq analysis for the identification of housekeeping genes useful for normalization of gene expression values during Striga hermonthica development.
Article in Molecular biology reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Neofunctionalized RGF pathways drive haustorial organogenesis in parasitic plants.Science advances · 2025Article
- Striga hermonthica induces lignin deposition at the root tip to facilitate prehaustorium formation and obligate parasitism.Plant communications · 2025Article
- Effect of Trichomonacide 6-Nitro-1International journal of molecular sciences · 2024Article
- Genome-enabled discovery of candidate virulence loci in Striga hermonthica, a devastating parasite of African cereal crops.The New phytologist · 2022Article
- Plant science's next top models.Annals of botany · 2020Review
- Genome-wide identification of internal reference genes for normalization of gene expression values during endosperm development in wheat.Journal of applied genetics · 2019Article
- An explanatory model of temperature influence on flowering through whole-plant accumulation ofIn silico plants · 2019Article
- Phloem-Conducting Cells in Haustoria of the Root-Parasitic Plant Phelipanche aegyptiaca Retain Nuclei and Are Not Mature Sieve Elements.Plants (Basel, Switzerland) · 2017Article
- Quinone oxidoreductase 2 is involved in haustorium development of the parasitic plant Phtheirospermum japonicum.Plant signaling & behavior · 2017Article
- Comparative physiological and transcriptomic analyses provide integrated insight into osmotic, cold, and salt stress tolerance mechanisms in banana.Scientific reports · 2017Article
- Selection and validation of appropriate reference genes for quantitative real-time PCR analysis in Salvia hispanica.PloS one · 2017Article
- Article
- Genome-Wide Constitutively Expressed Gene Analysis and New Reference Gene Selection Based on Transcriptome Data: A Case Study from Poplar/Canker Disease Interaction.Frontiers in plant science · 2017Article
- Article
- LbCML38 and LbRH52, two reference genes derived from RNA-Seq data suitable for assessing gene expression in Lycium barbarum L.Scientific reports · 2016Article
- A single amino acid mutation ofBreeding science · 2016Article
- Structural basis of unique ligand specificity of KAI2-like protein from parasitic weed Striga hermonthica.Scientific reports · 2016Article
- Evaluation of Sorghum [Sorghum bicolor (L.)] Reference Genes in Various Tissues and under Abiotic Stress Conditions for Quantitative Real-Time PCR Data Normalization.Frontiers in plant science · 2016Article
- Comparative transcriptome analyses reveal core parasitism genes and suggest gene duplication and repurposing as sources of structural novelty.Molecular biology and evolution · 2015Article
- Identification of internal reference genes for gene expression normalization between the two sexes in dioecious white Campion.PloS one · 2014Article
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9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Striga is a root parasitic weed that attacks many of the staple crops in Africa, India and Southeast Asia, inflicting tremendous losses in yield and for which there are few effective control measures. Studies of parasitic plant virulence and host resistance will be greatly facilitated by the recent emergence of genomic resources that include extensive transcriptome sequence datasets spanning all life stages of S. hermonthica. Functional characterization of Striga genes will require detailed analyses of gene expression patterns. Quantitative real-time PCR is a powerful tool for quantifying gene expression, but correct normalization of expression levels requires identification of control genes that have stable expression across tissues and life stages. Since no S. hermonthica housekeeping genes have been established for this purpose, we evaluated the suitability of six candidate housekeeping genes across key life stages of S. hermonthica from seed conditioning to flower initiation using qRT-PCR and high-throughput cDNA sequencing. Based on gene expression analysis by qRT-PCR and RNA-Seq across heterogeneous Striga life stages, we determined that using the combination of three genes, UBQ1, PP2A and TUB1 provides the best normalization for gene expression throughout the parasitic life cycle. The housekeeping genes characterized here provide robust standards that will facilitate powerful descriptions of parasite gene expression patterns.
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