ArticleMethods (San Diego, Calif.)2013
A comparison of binding surfaces for SPR biosensing using an antibody-antigen system and affinity distribution analysis.
Article in Methods (San Diego, Calif.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Curbing Autoimmunity: A New Fab Fragment Targeting CD40-CD40L Halts B-Cell Activation and Differentiation.European journal of immunology · 2026Article
- In-Solution Characterization of Biomolecular Interaction Kinetics under Native Conditions.Analytical chemistry · 2025Article
- Single Molecule Kinetic Fingerprinting of Glycans on IgA1 Antibodies.Analytical chemistry · 2025Article
- Optimizing Stability and Performance of Silver-Based Grating Structures for Surface Plasmon Resonance Sensors.Sensors (Basel, Switzerland) · 2023Article
- Lymphocyte activation gene 3 is increased and affects cytokine production in rheumatoid arthritis.Arthritis research & therapy · 2023Article
- Antigen footprint governs activation of the B cell receptor.Nature communications · 2023Article
- Galectin-3 interacts with PD-1 and counteracts the PD-1 pathway-driven regulation of T cell and osteoclast activity in Rheumatoid Arthritis.Scandinavian journal of immunology · 2023Article
- Structures of synthetic nanobody-SARS-CoV-2 receptor-binding domain complexes reveal distinct sites of interaction.The Journal of biological chemistry · 2021Article
- Label-free methods for opticalPhysical chemistry chemical physics : PCCP · 2021Review
- Impedimetric and Plasmonic Sensing of Collagen I Using a Half-Antibody-Supported, Au-Modified, Self-Assembled Monolayer System.Biosensors · 2021Article
- Structures of synthetic nanobody-SARS-CoV-2-RBD complexes reveal distinct sites of interaction and recognition of variants.Research square · 2021Article
- Recent Advancements in Receptor Layer Engineering for Applications in SPR-Based Immunodiagnostics.Sensors (Basel, Switzerland) · 2021Review
- Bulk-Effect-Free Method for Binding Kinetic Measurements Enabling Small-Molecule Affinity Characterization.ACS omega · 2021Article
- SPRD: a surface plasmon resonance database of common factors for better experimental planning.BMC molecular and cell biology · 2021Article
- Synthetic nanobody-SARS-CoV-2 receptor-binding domain structures identify distinct epitopes.bioRxiv : the preprint server for biology · 2021Article
- Kinetic analysis of antibody binding to integral membrane proteins stabilized in SMALPs.BBA advances · 2021Article
- Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers.Scientific reports · 2020Article
- Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association.Nature communications · 2020Article
- Non-Langmuir Kinetics of DNA Surface Hybridization.Biophysical journal · 2020Article
- Significance Testing and Multivariate Analysis of Datasets from Surface Plasmon Resonance and Surface Acoustic Wave Biosensors: Prediction and Assay Validation for Surface Binding of Large Analytes.Sensors (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The application of optical biosensors in the study of macromolecular interactions requires immobilization of one binding partner to the surface. It is often highly desirable that the immobilization is uniform and does not affect the thermodynamic and kinetic binding parameters to soluble ligands. To achieve this goal, a variety of sensor surfaces, coupling strategies and surface chemistries are available. Previously, we have introduced a technique for determining the distribution of affinities and kinetic rate constants from families of binding and dissociation traces acquired at different concentrations of soluble ligand. In the present work, we explore how this affinity distribution analysis can be useful in the assessment and optimization of surface immobilization. With this goal, using an antibody-antigen interaction as a model system, we study the activity, thermodynamic and kinetic binding parameters, and heterogeneity of surface sites produced with different commonly used sensor surfaces, at different total surface densities and with direct immobilization or affinity capture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.