ArticleBMC genomics2012
Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation.
Article in BMC genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
77 citing papers in PubMed, 135 citations in OpenAlex.
- Comparative analysis of microRNAs in bovine colostrum and neonatal calf blood.Journal of the science of food and agriculture · 2026Article
- Differential Expression of MicroRNAs in the Mammary Gland of Lactating and Dry Murciano-Granadina Goats.Animals : an open access journal from MDPI · 2026Article
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- Profiling several key milk miRNAs and analysing their signalling pathways in dairy sheep breeds during peak and late lactation.Veterinary medicine and science · 2024Article
- Hormonal regulation of miRNA during mammary gland development.Biology open · 2024Article
- Associations between human milk EV-miRNAs and oligosaccharide concentrations in human milk.Frontiers in immunology · 2024Article
- miRNA-protein-metabolite interaction network reveals the regulatory network and players of pregnancy regulation in dairy cows.Frontiers in cell and developmental biology · 2024Review
- Pasteurization of human milk affects the miRNA cargo of EVs decreasing its immunomodulatory activity.Scientific reports · 2023Article
- miR-497 RegulatesGenes · 2023Article
- Human Milk-Derived Levels of let-7g-5p May Serve as a Diagnostic and Prognostic Marker of Low Milk Supply in Breastfeeding Women.Nutrients · 2023Article
- MicroRNA-200c Affects Milk Fat Synthesis by TargetingInternational journal of molecular sciences · 2022Article
- Comparative Study of the Expression Profiles of miRNAs of Milk-Derived Exosomes of Yak and Jeryak.Animals : an open access journal from MDPI · 2022Article
- Variation of miRNA Content in Cow Raw Milk Depending on the Dairy Production System.International journal of molecular sciences · 2022Article
- An Integrated Analysis of Lactation-Related miRNA and mRNA Expression Profiles in Donkey Mammary Glands.Genes · 2022Article
- Emerging Roles of Noncoding RNAs in Bovine Mastitis Diseases.Pathogens (Basel, Switzerland) · 2022Review
- Nutritional Modulation, Gut, and Omics Crosstalk in Ruminants.Animals : an open access journal from MDPI · 2022Review
- Mammary epithelial cell transcriptome reveals potential roles of lncRNAs in regulating milk synthesis pathways in Jersey and Kashmiri cattle.BMC genomics · 2022Article
- The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep.Non-coding RNA · 2021Review
- Regulation of Milk Protein Synthesis by Free and Peptide-Bound Amino Acids in Dairy Cows.Biology · 2021Review
17 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroRNAs (miRNAs) have been implicated in the regulation of milk protein synthesis and development of the mammary gland (MG). However, the specific functions of miRNAs in these regulations are not clear. Therefore, the elucidation of miRNA expression profiles in the MG is an important step towards understanding the mechanisms of lactogenesis.
resultsTwo miRNA libraries were constructed from MG tissues taken from a lactating and a non-lactating Holstein dairy cow, respectively, and the short RNA sequences (18-30 nt) in these libraries were sequenced by Solexa sequencing method. The libraries included 885 pre-miRNAs encoding for 921 miRNAs, of which 884 miRNAs were unique sequences and 544 (61.5%) were expressed in both periods. A custom-designed microarray assay was then performed to compare miRNA expression patterns in the MG of lactating and non-lactating dairy cows. A total of 56 miRNAs in the lactating MG showed significant differences in expression compared to non-lactating MG (P<0.05). Integrative miRNA target prediction and network analysis approaches were employed to construct an interaction network of lactation-related miRNAs and their putative targets. Using a cell-based model, six miRNAs (miR-125b, miR-141, miR-181a, miR-199b, miR-484 and miR-500) were studied to reveal their possible biological significance.
conclusionOur study provides a broad view of the bovine MG miRNA expression profile characteristics. Eight hundred and eighty-four miRNAs were identified in bovine MG. Differences in types and expression levels of miRNAs were observed between lactating and non-lactating bovine MG. Systematic predictions aided in the identification of lactation-related miRNAs, providing insight into the types of miRNAs and their possible mechanisms in regulating lactation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.