ArticleFrontiers in plant science2012
The potato mop-top virus TGB2 protein and viral RNA associate with chloroplasts and viral infection induces inclusions in the plastids.
Article in Frontiers in plant science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Expanding the geographic and host range of moss-nostocacean symbioses in central Europe.Oecologia · 2026Article
- To win the battle: the chloroplast is a key battleground in plant-pathogen interactions.Horticulture research · 2026Article
- Investigating the Roles of Coat Protein and Triple Gene Block Proteins of Potato Mop-Top Virus Using a Heterologous Expression System.International journal of molecular sciences · 2024Article
- Article
- The Crucial Role of Chloroplast-Related Proteins in Viral Genome Replication and Host Defense against Positive-Sense Single-Stranded RNA Viruses.The plant pathology journal · 2023Review
- NSvc4 Encoded by Rice Stripe Virus Targets Host Chloroplasts to Suppress Chloroplast-Mediated Defense.Viruses · 2021Article
- Three-dimensional quantitative imaging of Tobacco mosaic virus and Zucchini yellow mosaic virus induced ultrastructural changes.Protoplasma · 2021Review
- Comprehensive Proteomic Analysis of Lysine Acetylation inFrontiers in microbiology · 2021Article
- Autophagy is involved in assisting the replication of Bamboo mosaic virus in Nicotiana benthamiana.Journal of experimental botany · 2019Article
- The Potato Virus X TGBp2 Protein Plays Dual Functional Roles in Viral Replication and Movement.Journal of virology · 2019Article
- Proteomic and Phosphoproteomic Analysis in Tobacco Mosaic Virus-Infected Tobacco (Nicotiana tabacum).Biomolecules · 2019Article
- Recent trends in click chemistry as a promising technology for virus-related research.Virus research · 2018Review
- Chloroplast: the Trojan horse in plant-virus interaction.Molecular plant pathology · 2018Review
- The Role of the Chloroplast in the Replication of Positive-Sense Single-Stranded Plant RNA Viruses.Frontiers in plant science · 2018Article
- Three-Dimensional Analysis of Chloroplast Structures Associated with Virus Infection.Plant physiology · 2018Article
- Chloroplast in Plant-Virus Interaction.Frontiers in microbiology · 2016Review
- Chloroplast phosphoglycerate kinase is involved in the targeting of Bamboo mosaic virus to chloroplasts in Nicotiana benthamiana plants.Plant physiology · 2013Article
- e-Book on plant virus infection-a cell biology perspective.Frontiers in plant science · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The potato mop-top virus (PMTV) triple gene block 2 (TGB2) movement proteins fused to monomeric red fluorescent protein (mRFP-TGB2) was expressed under the control of the PMTV subgenomic promoter from a PMTV vector. The subcellular localizations and interactions of mRFP-TGB2 were investigated using confocal imaging [confocal laser-scanning microscope, (CLSM)] and biochemical analysis. The results revealed associations with membranes of the endoplasmic reticulum (ER), mobile granules, small round structures (1-2 μm in diameter), and chloroplasts. Expression of mRFP-TGB2 in epidermal cells enabled cell-to-cell movement of a TGB2 defective PMTV reporter clone, indicating that the mRFP-TGB2 fusion protein was functional and required for cell-to-cell movement. Protein-lipid interaction assays revealed an association between TGB2 and lipids present in chloroplasts, consistent with microscopical observations where the plastid envelope was labeled later in infection. To further investigate the association of PMTV infection with chloroplasts, ultrastructural studies of thin sections of PMTV-infected potato and Nicotiana benthamiana leaves by electron microscopy revealed abnormal chloroplasts with cytoplasmic inclusions and terminal projections. Viral coat protein (CP), genomic RNA and fluorescently-labeled TGB2 were detected in plastid preparations isolated from the infected leaves, and viral RNA was localized to chloroplasts in infected tissues. The results reveal a novel association of TGB2 and vRNA with chloroplasts, and suggest viral replication is associated with chloroplast membranes, and that TGB2 plays a novel role in targeting the virus to chloroplasts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.