ArticleProceedings of the National Academy of Sciences of the United States of America2013
Activation of NF-κB signalling by fusicoccin-induced dimerization.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Competitive protein recruitment in artificial cells.Communications chemistry · 2024Article
- Small molecules targeting protein-protein interactions for cancer therapy.Acta pharmaceutica Sinica. B · 2023Review
- Switchable Control of Scaffold Protein Activity via Engineered Phosphoregulated Autoinhibition.ACS synthetic biology · 2022Article
- Probing the 14-3-3 Isoform-Specificity Profile of Protein-Protein Interactions Stabilized by Fusicoccin A.ACS omega · 2020Article
- Designed Asymmetric Protein Assembly on a Symmetric Scaffold.Angewandte Chemie (International ed. in English) · 2020Article
- Conjugated Protein Domains as Engineered Scaffold Proteins.Bioconjugate chemistry · 2020Review
- Modular bioengineered kinase sensorsChemical science · 2020Article
- 14-3-3 proteins protect AMPK-phosphorylated ten-eleven translocation-2 (TET2) from PP2A-mediated dephosphorylation.The Journal of biological chemistry · 2020Article
- Protease-Activatable Scaffold Proteins as Versatile Molecular Hubs in Synthetic Signaling Networks.ACS synthetic biology · 2018Article
- Self-Reporting Chemically Induced Protein Proximity System Based on a Malachite Green Derivative and the L5** Fluorogen Activating Protein.Bioconjugate chemistry · 2018Article
- A chemically induced proximity system engineered from the plant auxin signaling pathway.Chemical science · 2018Article
- Modular access to functionalized 5-8-5 fused ring systemsChemical science · 2018Article
- Recent trends in the structural revision of natural products.Natural product reports · 2018Review
- Synthesis and biological evaluation of rapamycin-derived, next generation small molecules.MedChemComm · 2018Review
- Identification of Two Secondary Ligand Binding Sites in 14-3-3 Proteins Using Fragment Screening.Biochemistry · 2017Article
- Small-Molecule-Induced and Cooperative Enzyme Assembly on a 14-3-3 Scaffold.Chembiochem : a European journal of chemical biology · 2017Article
- Chemical Proteomics for Target Discovery of Head-to-Tail Cyclized Mini-Proteins.Frontiers in chemistry · 2017Article
- Flipping the Switch on Clathrin-Mediated Endocytosis using Thermally Responsive Protein Microdomains.Advanced functional materials · 2014Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chemically induced dimerization is an important tool in chemical biology for the analysis of protein function in cells. Here we report the use of the natural product fusicoccin (FC) to induce dimerization of 14-3-3-fused target proteins with proteins tagged to the C terminus (CT) of the H(+)-ATPase PMA2. To prevent nonproductive or detrimental interactions of the 14-3-3 proteins and CT fusions with endogenous cell proteins, their interaction surface was engineered to facilitate FC-induced dimerization exclusively between the introduced protein constructs. Live-cell imaging documented the reversible FC-induced translocation of 14-3-3 and CT to different cell compartments depending on localization sequences fused to their dimerization partner protein. The functionality of this system was demonstrated by the FC-induced importation of the NF-κB-CT into the nucleus. In HeLa cells, FC-mediated dimerization of the NF-κB-CT with a constitutively nuclear-localized 14-3-3 protein led to an NF-κB-specific cellular response by inducing IL-8 secretion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.