ArticleJournal of clinical microbiology2013
Comparing pulsed-field gel electrophoresis with multilocus sequence typing, spa typing, staphylococcal cassette chromosome mec (SCCmec) typing, and PCR for panton-valentine leukocidin, arcA, and opp3 in methicillin-resistant Staphylococcus aureus isolates at a U.S. Medical Center.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
54 citing papers in PubMed, 78 citations in OpenAlex.
- Post-discharge decolonization of patients harboring methicillin-resistantInfection control and hospital epidemiology · 2023Trial
- Impact of Staphylococcus aureus USA300 Colonization and Skin Infections on Systemic Immune Responses in Humans.Journal of immunology (Baltimore, Md. : 1950) · 2016Trial
- CharacterizingmSystems · 2025Article
- Multi-strain carriage and intrahost diversity ofMicrobial genomics · 2025Article
- Prevalence of methicillin-resistant Staphylococcus aureus (MRSA) harboring mupirocin and biocide resistance genes in a large health care system.American journal of infection control · 2025Article
- Historic methicillin-resistant Staphylococcus aureus: expanding current knowledge using molecular epidemiological characterization of a Swiss legacy collection.Genome medicine · 2024Article
- Risk Factors and Outcomes of Hematogenous Vertebral Osteomyelitis in Patients With Staphylococcus aureus Bacteremia.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2023Article
- SarS Is a Repressor of Staphylococcus aureus Bicomponent Pore-Forming Leukocidins.Infection and immunity · 2023Article
- Black and White Patients With Staphylococcus aureus Bacteremia Have Similar Outcomes but Different Risk Factors.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2023Article
- MRSA lineage USA300 isolated from bloodstream infections exhibit altered virulence regulation.Cell host & microbe · 2023Article
- Phage KViruses · 2022Article
- Sequence type 8 as an emerging clone of methicillin-resistantEmerging microbes & infections · 2021Article
- Genomic Update of Phenotypic Prediction Rule for Methicillin-Resistant Staphylococcus aureus (MRSA) USA300 Discloses Jail Transmission Networks with Increased Resistance.Microbiology spectrum · 2021Article
- Genomic Insights into Methicillin-Resistant Staphylococcus aureusApplied and environmental microbiology · 2021Article
- Characterization ofInfection and drug resistance · 2020Article
- Changing Characteristics of Staphylococcus aureus Bacteremia: Results From a 21-Year, Prospective, Longitudinal Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
- Carriage of Methicillin-resistantComparative medicine · 2019Article
- Association of Primate Veterinarians Guidelines For Mrsa Infections in Nonhuman Primates in Biomedical Research.Journal of the American Association for Laboratory Animal Science : JAALAS · 2019Article
- Methicillin-resistant Staphylococcus aureus: an overview of basic and clinical research.Nature reviews. Microbiology · 2019Review
- Sequential evolution of virulence and resistance during clonal spread of community-acquired methicillin-resistantProceedings of the National Academy of Sciences of the United States of America · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) has become a common cause of skin infections and invasive infections in community dwellers in the United States since the late 1990s. Isolates characterized as USA300 by pulsed-field gel electrophoresis (PFGE) are the predominant strain type in these infections. USA100 and USA500 strains commonly cause health care-associated infections. We compared PFGE with a number of other methods of genotyping in a sample of 149 clinical MRSA isolates from the University of Chicago Medical Center. The 5 USA500 isolates yielded 3 spa types and 2 multilocus sequence types (MLSTs). Among the 24 USA100 isolates, 21 (88%) were of spa type t002, 19 (79%) were of ST5, 2 carried arcA and opp3, and 1 was Panton-Valentine leukocidin positive (PVL(+)). Among the 102 USA300 isolates, 96 (94%) were of ST8 and 94 (92%) were of spa type t008. The combination of traits that provided the best sensitivity (98%), specificity (97%), positive predictive value (PPV) (99%), and negative predictive value (NPV) (95%) for identifying USA300 isolates were the presence of the arcA gene and the presence of the PVL genes (area under the curve, 0.980; 95% confidence interval [CI], 0.955 to 1.0). PFGE did not delineate a homogeneous group of MRSA genetic backgrounds, as documented for other typing methods, particularly for USA500 and USA100 pulsotypes. Documenting the presence of arcA and PVL genes by PCR was an efficient and accurate means of identifying USA300 in a collection of MRSA isolates in which USA300 is common. None of the tested genotyping methods provided an accurate means of identifying the next most common PFGE-based backgrounds, USA100 and USA500.
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