Evidence map›Paper›PMID 23269729›Full record

ArticleJournal of clinical microbiology2013

Multiplex real-time PCR assay for detection of methicillin-resistant Staphylococcus aureus (MRSA) strains suitable in regions of high MRSA endemicity.

Jeong-Uk Kim, Choong-Hwan Cha, Hae-Kyong An, Ho-Jun Lee, Mi-Na Kim

Abstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Evolving Rapid Methicillin-resistantJournal of global infectious diseases
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jeong-Uk KimDepartment of Laboratory Medicine, Gangneung Asan Hospital, University of Ulsan College of Medicine, Gangneung, South Korea. jukim@gnah.co.kr
Choong-Hwan Cha
Hae-Kyong An
Ho-Jun Lee
Mi-Na Kim

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A multiplex real-time PCR assay that simultaneously detects the mecA, staphylococcal cassette chromosome (SCCmec)-open reading frame X (orfX) junction, and staphylococcal 16S rRNA genes was developed and evaluated using 444 staphylococcal strains. We demonstrated that this assay resulted in fewer false-positive results than a single-locus real-time PCR assay that amplified the SCCmec-orfX junction. This assay would be useful in a clinical laboratory in a region of high endemicity for methicillin-resistant Staphylococcus aureus (MRSA) infections.

Indexed as

Bacterial ProteinsBacteriological TechniquesFalse Positive ReactionsHumansMethicillin-Resistant Staphylococcus aureusMolecular Diagnostic TechniquesMultiplex Polymerase Chain ReactionPenicillin-Binding ProteinsReal-Time Polymerase Chain ReactionRNA, Ribosomal, 16SSensitivity and SpecificityStaphylococcal InfectionsBacterial ProteinsmecA protein, Staphylococcus aureusPenicillin-Binding ProteinsRNA, Ribosomal, 16S

Identifiers

PMID23269729
PMCPMC3592057

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.