ArticleAmerican journal of physiology. Renal physiology2013
Altered V-ATPase expression in renal intercalated cells isolated from B1 subunit-deficient mice by fluorescence-activated cell sorting.
Article in American journal of physiology. Renal physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 32 citations in OpenAlex.
- WDR72 Is Required for Urinary Acidification and Normal HActa physiologica (Oxford, England) · 2026Article
- Dmxl1 Is an Essential Mammalian Gene that Is Required for V-ATPase Assembly and Function In Vivo.Function (Oxford, England) · 2024Article
- Generation ofAmerican journal of physiology. Renal physiology · 2023Article
- A non-dividing cell population with high pyruvate dehydrogenase kinase activity regulates metabolic heterogeneity and tumorigenesis in the intestine.Nature communications · 2022Article
- The evolutionary conserved TLDc domain defines a new class of (HScientific reports · 2021Article
- Changes in the expression of the B subunit of vacuolar HIranian journal of basic medical sciences · 2021Article
- Circulating testican-2 is a podocyte-derived marker of kidney health.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Roles of vacuolar HInternational journal of clinical and experimental pathology · 2020Article
- Integrin-Linked Kinase Deficiency in Collecting Duct Principal Cell Promotes Necroptosis of Principal Cell and Contributes to Kidney Inflammation and Fibrosis.Journal of the American Society of Nephrology : JASN · 2019Article
- Unravelling purinergic regulation in the epididymis: activation of V-ATPase-dependent acidification by luminal ATP and adenosine.The Journal of physiology · 2019Article
- Whole Transcriptome Analysis of Renal Intercalated Cells Predicts Lipopolysaccharide Mediated Inhibition of Retinoid X Receptor alpha Function.Scientific reports · 2019Article
- Sex-dependent differences in water homeostasis in wild-type and V-ATPase B1-subunit deficient mice.PloS one · 2019Article
- Megalin mediates plasma membrane to mitochondria cross-talk and regulates mitochondrial metabolism.Cellular and molecular life sciences : CMLS · 2018Article
- Cell-specific qRT-PCR of renal epithelial cells reveals a novel innate immune signature in murine collecting duct.American journal of physiology. Renal physiology · 2018Article
- Targeted deletion of the Ncoa7 gene results in incomplete distal renal tubular acidosis in mice.American journal of physiology. Renal physiology · 2018Article
- Extracellular Adenosine Stimulates Vacuolar ATPase-Dependent Proton Secretion in Medullary Intercalated Cells.Journal of the American Society of Nephrology : JASN · 2018Article
- Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice.American journal of physiology. Renal physiology · 2018Article
- Transcriptomes of major renal collecting duct cell types in mouse identified by single-cell RNA-seq.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Deletion of β1-integrin in collecting duct principal cells leads to tubular injury and renal medullary fibrosis.American journal of physiology. Renal physiology · 2017Article
- Ezrin directly interacts with AQP2 and promotes its endocytosis.Journal of cell science · 2017Article
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
Unlike human patients with mutations in the 56-kDa B1 subunit isoform of the vacuolar proton-pumping ATPase (V-ATPase), B1-deficient mice (Atp6v1b1(-/-)) do not develop metabolic acidosis under baseline conditions. This is due to the insertion of V-ATPases containing the alternative B2 subunit isoform into the apical membrane of renal medullary collecting duct intercalated cells (ICs). We previously reported that quantitative Western blots (WBs) from whole kidneys showed similar B2 protein levels in Atp6v1b1(-/-) and wild-type mice (Păunescu TG, Russo LM, Da Silva N, Kovacikova J, Mohebbi N, Van Hoek AN, McKee M, Wagner CA, Breton S, Brown D. Am J Physiol Renal Physiol 293: F1915-F1926, 2007). However, WBs from renal medulla (including outer and inner medulla) membrane and cytosol fractions reveal a decrease in the levels of the ubiquitous V-ATPase E1 subunit. To compare V-ATPase expression specifically in ICs from wild-type and Atp6v1b1(-/-) mice, we crossed mice in which EGFP expression is driven by the B1 subunit promoter (EGFP-B1(+/+) mice) with Atp6v1b1(-/-) mice to generate novel EGFP-B1(-/-) mice. We isolated pure IC populations by fluorescence-assisted cell sorting from EGFP-B1(+/+) and EGFP-B1(-/-) mice to compare their V-ATPase subunit protein levels. We report that V-ATPase A, E1, and H subunits are all significantly downregulated in EGFP-B1(-/-) mice, while the B2 protein level is considerably increased in these animals. We conclude that under baseline conditions B2 upregulation compensates for the lack of B1 and is sufficient to maintain basal acid-base homeostasis, even when other V-ATPase subunits are downregulated.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.