ArticleAnalytical chemistry2013
Single domain antibodies for the detection of ricin using silicon photonic microring resonator arrays.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- On-chip multivariant COVID 19 photonic sensor based on silicon nitride double-microring resonators.Nanophotonics (Berlin, Germany) · 2023Article
- Biofunctionalization of Multiplexed Silicon Photonic Biosensors.Biosensors · 2022Review
- An Optimization Framework for Silicon Photonic Evanescent-Field Biosensors Using Sub-Wavelength Gratings.Biosensors · 2022Article
- Rapid detection of an Ebola biomarker with optical microring resonators.Cell reports methods · 2022Article
- Applications of nanobodies in plant science and biotechnology.Plant molecular biology · 2021Review
- Recent advances in environmental and clinical analysis using microring resonator-based sensors.Current opinion in environmental science & health · 2019Article
- Optical Biosensors Based on Silicon-On-Insulator Ring Resonators: A Review.Molecules (Basel, Switzerland) · 2019Review
- Article
- Applications of Optical Microcavity Resonators in Analytical Chemistry.Annual review of analytical chemistry (Palo Alto, Calif.) · 2016Article
- An Electrochemiluminescence Immunosensor Based on Gold-Magnetic Nanoparticles and Phage Displayed Antibodies.Sensors (Basel, Switzerland) · 2016Article
- Development and validation of an immunosensor for monocyte chemotactic protein 1 using a silicon photonic microring resonator biosensing platform.Clinical biochemistry · 2016Article
- Magnetically-actuated, bead-enhanced silicon photonic immunosensor.Analytical methods : advancing methods and applications · 2015Article
- Whispering gallery mode sensors.Advances in optics and photonics · 2015Article
- Refractive index-based detection of gradient elution liquid chromatography using chip-integrated microring resonator arrays.Analytical chemistry · 2014Article
- Fluorescent labelling of the actin cytoskeleton in plants using a cameloid antibody.Plant methods · 2014Article
- Biomolecular analysis with microring resonators: applications in multiplexed diagnostics and interaction screening.Current opinion in chemical biology · 2013Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Ricin is a lethal protein toxin derived from the castor bean plant. Given its notorious history as a biowarfare agent and homicidal weapon, ricin has been classified as a category B bioterrorism agent. Current ricin detection methods based on immunoassays lack the required sensitivity and specificity for many homeland security surveillance applications. Importantly, many conventional antibody-based methodologies are unable to distinguish ricin from RCA 120, a nontoxic protein also found in the castor bean plant. Single domain antibodies (sdAbs), which are recombinantly derived from immunized llamas, are known to have high affinities for ricin A or B chains and low cross-reactivity with RCA 120. Herein, we demonstrate the use of silicon photonic microring resonators for antibody affinity profiling and one-step ricin detection at concentrations down to 300 pM using a 15 min, label-free assay format. These sdAbs were also simultaneously compared with a commercial anti-RCA IgG antibody in a multicapture agent, single target immunoassay using arrays of microrings, which allowed direct comparison of sensitivity and specificity. A selected sdAb was also found to exhibit outstanding specificity against another biotoxin, saporin, which has mechanism of action similar to ricin. Given the rapidity, scalability, and multiplexing capability of this silicon-based technology, this work represents a step toward using microring resonator arrays for the sensitive and specific detection of biowarfare agents.
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Registered trials
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