ArticleFrontiers in cellular and infection microbiology2012
Detection of Shiga toxin-producing Escherichia coli in ground beef using the GeneDisc real-time PCR system.
Article in Frontiers in cellular and infection microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Recent Advancements in Technologies to Detect EnterohaemorrhagicJournal of microbiology and biotechnology · 2023Review
- The "Big Six": Hidden Emerging Foodborne Bacterial Pathogens.Tropical medicine and infectious disease · 2022Review
- Identification of Novel Biomarkers for Priority Serotypes of Shiga Toxin-ProducingFrontiers in microbiology · 2018Article
- Revisiting the STEC Testing Approach: Using espK and espV to Make Enterohemorrhagic Escherichia coli (EHEC) Detection More Reliable in Beef.Frontiers in microbiology · 2016Article
- Six Novel O Genotypes from Shiga Toxin-Producing Escherichia coli.Frontiers in microbiology · 2016Article
- Detection, Characterization, and Typing of Shiga Toxin-Producing Escherichia coli.Frontiers in microbiology · 2016Review
- Evaluation of enzyme immunoassays and real-time PCR for detecting Shiga toxin-producing Escherichia coli in Southern Alberta, Canada.Journal of clinical microbiology · 2015Article
- Article
- Draft Whole-Genome Sequences of Three Shiga Toxin-Producing Escherichia coli O91:H21 Isolates, Two from Hemolytic Uremic Syndrome Patients and One of Porcine Origin.Genome announcements · 2014Article
- Hemolytic uremic syndrome following infection with O111 Shiga toxin-producing Escherichia coli revealed through molecular diagnostics.Journal of clinical microbiology · 2014Article
- Shiga toxin-producing Escherichia coli in human, cattle, and foods. Strategies for detection and control.Frontiers in cellular and infection microbiology · 2014Article
- Advances in detection methods for Shiga toxin-producing Escherichia coli (STEC).Frontiers in microbiology · 2014Article
- Phenotypic and genotypic characterization of biofilm forming capabilities in non-O157 Shiga toxin-producing Escherichia coli strains.PloS one · 2013Article
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2 authors.
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Abstract
Escherichia coli O157:H7 and certain non-O157 Shiga toxin-producing Escherichia coli (STEC) serogroups have emerged as important public health threats. The development of methods for rapid and reliable detection of this heterogeneous group of pathogens has been challenging. GeneDisc real-time PCR assays were evaluated for detection of the stx(1), stx(2), eae, and ehxA genes and a gene that identifies the O157 serogroup followed by a second GeneDisc assay targeting serogroup-specific genes of STEC O26, O45, O91, O103, O111, O113, O121, O145, and O157. The ability to detect the STEC serogroups in ground beef samples artificially inoculated at a level of ca. 2-20 CFU/25 g and subjected to enrichment in mTSB or buffered peptone water (BPW) was similar. Following enrichment, all inoculated ground beef samples showed amplification of the correct set of target genes carried by each strain. Samples inoculated with STEC serogroups O26, O45, O103, O111, O121, O145, and O157 were subjected to immunomagnetic separation (IMS), and isolation was achieved by plating onto Rainbow agar O157. Colonies were confirmed by PCR assays targeting stx(1), stx(2), eae, and serogroup-specific genes. Thus, this work demonstrated that GeneDisc assays are rapid, sensitive, and reliable and can be used for screening ground beef and potentially other foods for STEC serogroups that are important food-borne pathogens worldwide.
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