ReviewPhilosophical transactions of the Royal Society of London. Series B, Biological sciences2013
Development of new photon-counting detectors for single-molecule fluorescence microscopy.
Review in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 117 citations in OpenAlex.
- Simulating Freely Diffusing Single-Molecule FRET Data with Consideration of Protein Conformational Dynamics.The journal of physical chemistry. B · 2026Article
- Choosing the Probe for Single-Molecule Fluorescence Microscopy.International journal of molecular sciences · 2022Review
- Integrative structural dynamics probing of the conformational heterogeneity in synaptosomal-associated protein 25.Cell reports. Physical science · 2021Article
- Fast Gating for Raman Spectroscopy.Sensors (Basel, Switzerland) · 2021Review
- Lightsheet fluorescence lifetime imaging microscopy with wide-field time-correlated single photon counting.Journal of biophotonics · 2020Article
- Wide-field time-correlated single photon counting-based fluorescence lifetime imaging microscopy.Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment · 2019Review
- High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins.Methods (San Diego, Calif.) · 2019Article
- Single-photon avalanche diode imagers in biophotonics: review and outlook.Light, science & applications · 2019Review
- Optical crosstalk in SPAD arrays for high-throughput single-molecule fluorescence spectroscopy.Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment · 2018Article
- Multifocal multiphoton volumetric imaging approach for high-speed time-resolved Förster resonance energy transfer imaging in vivo.Optics letters · 2018Article
- Dynamic action of the Sec machinery during initiation, protein translocation and termination.eLife · 2018Article
- Article
- Silicon single-photon avalanche diodes with nano-structured light trapping.Nature communications · 2017Article
- Development and characterization of an 8×8 SPAD-array module for gigacount per second applications.Proceedings of SPIE--the International Society for Optical Engineering · 2017Article
- Multispot single-molecule FRET: High-throughput analysis of freely diffusing molecules.PloS one · 2017Article
- Superconducting nanowire single-photon detectors with non-periodic dielectric multilayers.Scientific reports · 2016Article
- Silicon technologies for arrays of Single Photon Avalanche Diodes.Proceedings of SPIE--the International Society for Optical Engineering · 2016Article
- FRETBursts: An Open Source Toolkit for Analysis of Freely-Diffusing Single-Molecule FRET.PloS one · 2016Article
- A Method for Extracting the Free Energy Surface and Conformational Dynamics of Fast-Folding Proteins from Single Molecule Photon Trajectories.The journal of physical chemistry. B · 2015Article
- A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging.Biomedical optics express · 2015Article
Corrections and comments
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Authors and funding
22 authors at 3 institutions in 2 countries.
Funding
Abstract
Two optical configurations are commonly used in single-molecule fluorescence microscopy: point-like excitation and detection to study freely diffusing molecules, and wide field illumination and detection to study surface immobilized or slowly diffusing molecules. Both approaches have common features, but also differ in significant aspects. In particular, they use different detectors, which share some requirements but also have major technical differences. Currently, two types of detectors best fulfil the needs of each approach: single-photon-counting avalanche diodes (SPADs) for point-like detection, and electron-multiplying charge-coupled devices (EMCCDs) for wide field detection. However, there is room for improvements in both cases. The first configuration suffers from low throughput owing to the analysis of data from a single location. The second, on the other hand, is limited to relatively low frame rates and loses the benefit of single-photon-counting approaches. During the past few years, new developments in point-like and wide field detectors have started addressing some of these issues. Here, we describe our recent progresses towards increasing the throughput of single-molecule fluorescence spectroscopy in solution using parallel arrays of SPADs. We also discuss our development of large area photon-counting cameras achieving subnanosecond resolution for fluorescence lifetime imaging applications at the single-molecule level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.