ArticleProceedings of the National Academy of Sciences of the United States of America2013
Cell cycle-dependent deposition of CENP-A requires the Dos1/2-Cdc20 complex.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- CENP-A in cancer: from centromere identity to genome instability.Molecular biology reports · 2026Review
- Deciphering differences in DNA methylation and transcriptome profiles of oocytes from pigs with high and low developmental competence.Environmental epigenetics · 2025Article
- Single-cell and bulk RNA sequencing analysis reveals CENPA as a potential biomarker and therapeutic target in cancers.PloS one · 2025Article
- The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary.Nucleic acids research · 2022Article
- The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis.Communications biology · 2022Article
- Recent insights into mechanisms preventing ectopic centromere formation.Open biology · 2021Review
- Rbm10 facilitates heterochromatin assembly via the Clr6 HDAC complex.Epigenetics & chromatin · 2021Article
- Review
- Article
- Cell Cycle-Regulated Transcription of CENP-A by the MBF Complex Ensures Optimal Level of CENP-A for Centromere Formation.Genetics · 2019Article
- Heterochromatin and RNAi regulate centromeres by protecting CENP-A from ubiquitin-mediated degradation.PLoS genetics · 2018Article
- New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.PLoS genetics · 2018Article
- In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe.Methods in molecular biology (Clifton, N.J.) · 2018Review
- Antibody Pull-Down Experiments in Fission Yeast.Methods in molecular biology (Clifton, N.J.) · 2018Review
- Are all repeats created equal? Understanding DNA repeats at an individual level.Current genetics · 2017Review
- Condensin Promotes Position Effects within Tandem DNA Repeats via the RITS Complex.Cell reports · 2016Article
- Ectopic centromere nucleation by CENP--a in fission yeast.Genetics · 2014Article
- DNA replication components as regulators of epigenetic inheritance--lesson from fission yeast centromere.Protein & cell · 2014Review
- Rad51-Rad52 mediated maintenance of centromeric chromatin in Candida albicans.PLoS genetics · 2014Article
- CRL4-like Clr4 complex in Schizosaccharomyces pombe depends on an exposed surface of Dos1 for heterochromatin silencing.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Centromeric histone CENP-A, a variant of canonical histone H3, plays a central role in proper chromosome segregation. Loading of CENP-A at centromeres is cell cycle-regulated: parental CENP-A is deposited at centromeres during S phase, whereas newly synthesized CENP-A is deposited during later stages of the cell cycle. The mechanisms involved in deposition of CENP-A at centromeres during S phase remain poorly understood. In fission yeast, loading of CENP-A during S phase is regulated by the GATA-type factor, Ams2. Here we show that the Dos1/2-Cdc20 complex, previously characterized as a silencing complex essential for inheritance of H3K9 methylation during S phase, is also required for localization of CENP-A(cnp1) at centromeres at this stage. Disruption of Dos1 (also known as Raf1/Clr8/Cmc1), Dos2 (also known as Raf2/Clr7/Cmc2), or Cdc20, a DNA polymerase epsilon subunit, results in dissociation of CENP-A from centromeres and mislocalization of the protein to noncentromeric sites. All three mutants display spindle disorganization and mitotic defects. Inactivation of Dos1 or Cdc20 also results in accumulation of noncoding RNA transcripts from centromeric cores, a feature common to mutants affecting kinetochore integrity. We further find that Dos1 physically associates with Ams2 and is required for the association of Ams2 with centromeric cores during S phase. Finally, we show that Dos2 associates with centromeric cores during S phase and that its recruitment to centromeric cores depends on Cdc20. This study identifies a physical link between DNA replication and CENP-A assembly machinery and provides mechanistic insight into how CENP-A is faithfully inherited during S phase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.