ArticleProceedings of the National Academy of Sciences of the United States of America2013
Two methods for full-length RNA sequencing for low quantities of cells and single cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers, 1 of them a synthesis that pooled it.
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Who cites it
74 citing papers in PubMed, 1 synthesis or guideline pooled it, 115 citations in OpenAlex.
- Research progress on the application of single-cell sequencing in autoimmune diseases.Genes and immunity · 2023Pooled it
- Hybridization capture increases on-target nanopore sequencing of plant RNA tobamovirus- derived cDNA libraries.Frontiers in microbiology · 2026Article
- Biosynthesis of Camphane Volatile Terpenes inPlants (Basel, Switzerland) · 2025Article
- Simultaneous profiling of RNA isoforms and chromatin accessibility of single cells of human retinal organoids.Nature communications · 2024Article
- Identification and classification of the genomes of novel microviruses in poultry slaughterhouse.Frontiers in microbiology · 2024Article
- The Evolution of Single-Cell RNA Sequencing Technology and Application: Progress and Perspectives.International journal of molecular sciences · 2023Review
- Interfacing microfluidics with information-rich detection systems for cells, bioparticles, and molecules.Analytical and bioanalytical chemistry · 2022Review
- The role of serum amyloid A1 in the adipogenic differentiation of human adipose-derived stem cells basing on single-cell RNA sequencing analysis.Stem cell research & therapy · 2022Article
- Low-host double MDA workflow for uncultured ASFV positive blood and serum sample sequencing.Frontiers in veterinary science · 2022Article
- Review
- The Application of Single-Cell RNA Sequencing in Studies of Autoimmune Diseases: a Comprehensive Review.Clinical reviews in allergy & immunology · 2021Review
- LnCeCell: a comprehensive database of predicted lncRNA-associated ceRNA networks at single-cell resolution.Nucleic acids research · 2021Article
- Single-Cell Sequencing and Organoids: A Powerful Combination for Modelling Organ Development and Diseases.Reviews of physiology, biochemistry and pharmacology · 2021Review
- Alpha lipoic acid promotes development of hematopoietic progenitors derived from human embryonic stem cells by antagonizing ROS signals.Journal of leukocyte biology · 2020Article
- NUP98-HOXA10hd fusion protein sustains multi-lineage haematopoiesis of lineage-committed progenitors in transplant setting.Cell proliferation · 2020Article
- SingleCellSignalR: inference of intercellular networks from single-cell transcriptomics.Nucleic acids research · 2020Article
- In-depth serum virome analysis in patients with acute liver failure with indeterminate etiology.Archives of virology · 2020Article
- Geostatistical visualization of ecological interactions in tumors.Proceedings. IEEE International Conference on Bioinformatics and Biomedicine · 2019Article
- Article
- Subclonal cooperation drives metastasis by modulating local and systemic immune microenvironments.Nature cell biology · 2019Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
Abstract
The ability to determine the gene expression pattern in low quantities of cells or single cells is important for resolving a variety of problems in many biological disciplines. A robust description of the expression signature of a single cell requires determination of the full-length sequence of the expressed mRNAs in the cell, yet existing methods have either 3' biased or variable transcript representation. Here, we report our protocols for the amplification and high-throughput sequencing of very small amounts of RNA for sequencing using procedures of either semirandom primed PCR or phi29 DNA polymerase-based DNA amplification, for the cDNA generated with oligo-dT and/or random oligonucleotide primers. Unlike existing methods, these protocols produce relatively uniformly distributed sequences covering the full length of almost all transcripts independent of their sizes, from 1,000 to 10 cells, and even with single cells. Both protocols produced satisfactory detection/coverage of the abundant mRNAs from a single K562 erythroleukemic cell or a single dorsal root ganglion neuron. The phi29-based method produces long products with less noise, uses an isothermal reaction, and is simple to practice. The semirandom primed PCR procedure is more sensitive and reproducible at low transcript levels or with low quantities of cells. These methods provide tools for mRNA sequencing or RNA sequencing when only low quantities of cells, a single cell, or even degraded RNA are available for profiling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.