ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2013
Comprehensive approach to the quantitative analysis of mitochondrial phospholipids by HPLC-MS.
Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- Species-selective plasma metabolism drives lysophosphatidylcholine depletion after cardiac arrest.The Journal of biological chemistry · 2026Article
- Analytical challenges and opportunities in the extraction, separation, and identification of food phospholipids.Frontiers in nutrition · 2026Review
- Improved survival and neurological outcomes with lysophosphatidylcholine supplementation in severe cardiac arrest model.Frontiers in pharmacology · 2025Article
- Mitochondrial membrane model: Lipids, elastic properties, and the changing curvature of cardiolipin.Biophysical journal · 2023Article
- Advances in methods to analyse cardiolipin and their clinical applications.Trends in analytical chemistry : TRAC · 2022Article
- The role of lipids in exosome biology and intercellular communication: Function, analytics and applications.Traffic (Copenhagen, Denmark) · 2021Review
- A concise review on lipidomics analysis in biological samples.ADMET & DMPK · 2021Review
- Recommendations for good practice in MS-based lipidomics.Journal of lipid research · 2021Review
- Identification of unusual phospholipids from bovine heart mitochondria by HPLC-MS/MS.Journal of lipid research · 2020Article
- BAM15-mediated mitochondrial uncoupling protects against obesity and improves glycemic control.EMBO molecular medicine · 2020Article
- Lipidomics from sample preparation to data analysis: a primer.Analytical and bioanalytical chemistry · 2020Review
- Relative Ratios Enhance the Diagnostic Power of Phospholipids in Distinguishing Benign and Cancerous Ovarian Masses.Cancers · 2019Article
- Challenges and Inconsistencies in Using Lysophosphatidic Acid as a Biomarker for Ovarian Cancer.Cancers · 2019Article
- A rapid and adaptable lipidomics method for quantitative UPLC-mass spectrometric analysis of phosphatidylethanolamine and phosphatidylcholineAnalytical methods : advancing methods and applications · 2019Article
- The role of decreased cardiolipin and impaired electron transport chain in brain damage due to cardiac arrest.Neurochemistry international · 2018Article
- Comparing phospholipid profiles of mitochondria and whole tissue: Higher PUFA content in mitochondria is driven by increased phosphatidylcholine unsaturation.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2018Article
- Comprehensive analysis of phospholipids in the brain, heart, kidney, and liver: brain phospholipids are least enriched with polyunsaturated fatty acids.Molecular and cellular biochemistry · 2018Article
- Lipidomics: Prospects from a technological perspective.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- DHA-supplemented diet increases the survival of rats following asphyxia-induced cardiac arrest and cardiopulmonary bypass resuscitation.Scientific reports · 2016Article
- Molecular species composition of plant cardiolipin determined by liquid chromatography mass spectrometry.Journal of lipid research · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A normal-phase HPLC-MS method was established to analyze mitochondrial phospholipids quantitatively as well as qualitatively. An efficient extraction procedure and chromatographic conditions were developed using twelve standardized phospholipids and lysophospholipids. The chromatographic conditions provided physical separation of phospholipids by class, and efficient ionization allowed detection of low abundance phospholipids such as phosphatidylglycerol and monolysocardiolipin. The chromatographic separation of each class of phospholipid permitted qualitative identification of molecular species without interference from other classes. This is advantageous for mitochondrial lipidomics because the composition of mitochondrial phospholipids varies depending on tissue source, pathological condition, and nutrition. Using the method, seven classes of phospholipids (phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, cardiolipin, and monolysocardiolipin) were detected in rat heart and skeletal muscle mitochondria and all but phosphatidylserine were quantified. The concentration was calculated using standard curves with an internal standard generated for each class of phospholipid. The method was validated for intraday and interday variation and showed excellent reproducibility and accuracy. This new method, with each step documented, provides a powerful tool for accurate quantitation of phospholipids, a basic structural component of mitochondrial membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.