ArticleThe Journal of allergy and clinical immunology2013
Asthma-associated differences in microbial composition of induced sputum.
Article in The Journal of allergy and clinical immunology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 4 registered trials, which are not on this map. Cited by 206 papers, 4 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Guangzhou Institute of Respiratory Disease
Oral Probiotic Administration to Modulate the Airway Microbiome in Obese Asthmatic Subjects
Non-Eosinophilic Neutrophilic Asthma
Determining the Effect of Probiotics on Microbiome and Volatile Organic Compounds in Patients After Surgical Repair of Congenital Diaphragmatic Hernia
Who cites it
206 citing papers in PubMed, 4 syntheses or guidelines pooled it, 380 citations in OpenAlex.
- The respiratory microbiota alpha-diversity in chronic lung diseases: first systematic review and meta-analysis.Respiratory research · 2022Pooled it
- The composition of lung microbiome in lung cancer: a systematic review and meta-analysis.BMC microbiology · 2021Pooled it
- The Effects of Corticosteroids on the Respiratory Microbiome: A Systematic Review.Frontiers in medicine · 2021Pooled it
- The microbiome in allergic disease: Current understanding and future opportunities-2017 PRACTALL document of the American Academy of Allergy, Asthma & Immunology and the European Academy of Allergy and Clinical Immunology.The Journal of allergy and clinical immunology · 2017Guideline
- Distinct associations of sputum and oral microbiota with atopic, immunologic, and clinical features in mild asthma.The Journal of allergy and clinical immunology · 2020Trial
- Features of the bronchial bacterial microbiome associated with atopy, asthma, and responsiveness to inhaled corticosteroid treatment.The Journal of allergy and clinical immunology · 2017Trial
- Smoking Cessation and the Microbiome in Induced Sputum Samples from Cigarette Smoking Asthma Patients.PloS one · 2016Trial
- Role of oral bacteria composition and functional gene profiles in respiratory diseases.BMJ open respiratory research · 2026Article
- Lung microbiome predictors of epigenetic aging and potential associations with smoking and electronic cigarette use.Clinical epigenetics · 2026Article
- Article
- B.uniformis IM01-derived IAA alleviates asthma via AhR/NLRP3 pathways in mice.Cellular and molecular life sciences : CMLS · 2025Article
- Metagenome and metabolome study on inhaled corticosteroids in asthma patients with side effects.Journal of integrative bioinformatics · 2025Article
- A new approach for microbe-disease association prediction: incorporating representation learning of latent relationships.BMC medical informatics and decision making · 2025Article
- Oral microbiota and respiratory diseases: advances and perspectives.Clinical microbiology reviews · 2025Review
- The impact of environmental factors on respiratory tract microbiome and respiratory system diseases.European journal of medical research · 2025Review
- Effects of azithromycin on alleviating airway inflammation in asthmatic mice by regulating airway microbiota and metabolites.Microbiology spectrum · 2025Article
- Early-Life Host-Microbial Interactions and Asthma Development: A Lifelong Impact?Immunological reviews · 2025Review
- Positive sputum fungal culture, fungal sensitisation, and airway microbial diversity in asthmatic children.Medical mycology · 2025Article
- Burn inhalation injury and intubation with dexamethasone-eluting endotracheal tubes modulate local microbiome and alter airway inflammation.Frontiers in bioengineering and biotechnology · 2025Article
- Precision Medicine in Asthma: The Role of Biomarkers.ImmunoTargets and therapy · 2025Review
146 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundIt is increasingly evident that microbial colonization of the respiratory tract might have a role in the pathogenesis of asthma.
objectiveWe sought to characterize and compare the microbiome of induced sputum in asthmatic and nonasthmatic adults.
methodsInduced sputum samples were obtained from 10 nonasthmatic subjects and 10 patients with mild active asthma (8/10 were not using inhaled corticosteroids). Total DNA was extracted from sputum supernatants and amplified by using primers specific for the V6 hypervariable region of bacterial 16s rRNA. Samples were barcoded, and equimolar concentrations of 20 samples were pooled and sequenced with the 454 GS FLX sequencer. Sequences were assigned to bacterial taxa by comparing them with 16s rRNA sequences in the Ribosomal Database Project.
resultsAll sputum samples contained 5 major bacterial phyla: Firmicutes, Proteobacteria, Actinobacteria, Fusobacterium, and Bacteroidetes, with the first 3 phyla accounting for more than 90% of the total sequences. Proteobacteria were present in higher proportions in asthmatic patients (37% vs 15%, P < .001). In contrast, Firmicutes (47% vs 63%, P = .17) and Actinobacteria (10% vs 14%, P = .36) were found more frequently in samples from nonasthmatic subjects, although this was not statistically significant. Hierarchical clustering produced 2 significant clusters: one contained primarily asthmatic samples and the second contained primarily nonasthmatic samples. In addition, samples from asthmatic patients had greater bacterial diversity compared with samples from nonasthmatic subjects.
conclusionPatients with mild asthma have an altered microbial composition in the respiratory tract that is similar to that observed in patients with more severe asthma.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.