ArticleJournal of cell science2013
PtdIns(3,4,5)P3 is constitutively synthesized and required for spindle translocation during meiosis in mouse oocytes.
Article in Journal of cell science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Novel variants in PADI6 genes cause female infertility due to early embryo arrest.Journal of assisted reproduction and genetics · 2024Article
- An Update on Physiopathological Roles of Akt in the ReprodAKTive Mammalian Ovary.Life (Basel, Switzerland) · 2024Review
- Expression patterns and the roles of phosphatidylinositol phosphatases in testis†.Biology of reproduction · 2022Article
- Novel genetic variants of KHDC3L and other members of the subcortical maternal complex associated with Beckwith-Wiedemann syndrome or Pseudohypoparathyroidism 1B and multi-locus imprinting disturbances.Clinical epigenetics · 2022Article
- Maternal effect factors that contribute to oocytes developmental competence: an update.Journal of assisted reproduction and genetics · 2022Review
- Advances in the study of CDC42 in the female reproductive system.Journal of cellular and molecular medicine · 2022Review
- Single-cell transcriptome and cell-specific network analysis reveal the reparative effect of neurotrophin-4 in preantral follicles grown in vitro.Reproductive biology and endocrinology : RB&E · 2021Article
- Hormesis of low-dose inhibition of pAkt1 (Ser473) followed by a great increase of proline-rich inositol polyphosphate 5-phosphatase (PIPP) level in oocytes.In vitro cellular & developmental biology. Animal · 2021Article
- Dissecting the mechanisms of cell division.The Journal of biological chemistry · 2019Review
- Phosphoinositides, Major Actors in Membrane Trafficking and Lipid Signaling Pathways.International journal of molecular sciences · 2017Review
- ECAT1 is essential for human oocyte maturation and pre-implantation development of the resulting embryos.Scientific reports · 2016Article
- The subcortical maternal complex: multiple functions for one biological structure?Journal of assisted reproduction and genetics · 2016Review
- Decrease in expression of maternal effect gene Mater is associated with maternal ageing in mice.Molecular human reproduction · 2016Article
- Transducin-like enhancer of split-6 (TLE6) is a substrate of protein kinase A activity during mouse oocyte maturation.Biology of reproduction · 2014Article
- Specific deletion of Cdc42 does not affect meiotic spindle organization/migration and homologous chromosome segregation but disrupts polarity establishment and cytokinesis in mouse oocytes.Molecular biology of the cell · 2013Article
- The road to maturation: somatic cell interaction and self-organization of the mammalian oocyte.Nature reviews. Molecular cell biology · 2013Review
- Article
- Symmetry breaking and polarity establishment during mouse oocyte maturation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Prior to ovulation, mammalian oocytes complete their first meiotic division and arrest at metaphase II. During this marked asymmetric cell division, the meiotic spindle moves dramatically from the center of the oocyte to the cortex to facilitate segregation of half of its chromosomal content into the diminutive first polar body. Recent investigations have documented crucial roles for filamentous actin (F-actin) in meiotic spindle translocation. However, the identity of the upstream regulators responsible for these carefully orchestrated movements has remained elusive. Utilizing fluorescently tagged probes and time-lapse confocal microscopy, we document that phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5)P3] is constitutively synthesized with spatial and temporal dynamics similar to that of F-actin and Formin 2 (Fmn2). Blockage of PtdIns(3,4,5)P3 synthesis by LY294002, a specific inhibitor of phosphoinositide 3-kinase (PI3K), disrupts cytoplasmic F-actin organization and meiotic spindle migration to the cortex. F-actin nucleator Fmn2 and Rho GTPase Cdc42 play roles in mediating the effect of PtdIns(3,4,5)P3 on F-actin assembly. Moreover, the spatial and temporal dynamics of PtdIns(3,4,5)P3 is impaired by depletion of MATER or Filia, two oocyte proteins encoded by maternal effect genes. Thus, PtdIns(3,4,5)P3 is synthesized during meiotic maturation and acts upstream of Cdc42 and Fmn2, but downstream of MATER/Filia proteins to regulate the F-actin organization and spindle translocation to the cortex during mouse oocyte meiosis.
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Registered trials
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