ArticleJournal of cell science2013
Separate roles of PKA and EPAC in renal function unraveled by the optogenetic control of cAMP levels in vivo.
Article in Journal of cell science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Identification of Key Residues in Allosteric Signaling of Photoactivated Adenylyl Cyclase.bioRxiv : the preprint server for biology · 2026Article
- Multifaceted roles of Epac signaling in renal functions.The Biochemical journal · 2025Review
- Voltage-gated ion channels are expressed in the Malpighian tubules and anal papillae of the yellow fever mosquito (Aedes aegypti), and may regulate ion transport during salt and water imbalance.The Journal of experimental biology · 2024Article
- The V-type HProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Rap1 regulates apical contractility to allow embryonic morphogenesis without tissue disruption and acts in part via Canoe-independent mechanisms.Molecular biology of the cell · 2023Article
- LSM12-EPAC1 defines a neuroprotective pathway that sustains the nucleocytoplasmic RAN gradient.PLoS biology · 2020Article
- Dual Activation of cAMP Production Through Photostimulation or Chemical Stimulation.Methods in molecular biology (Clifton, N.J.) · 2020Article
- The Drosophila Malpighian tubule as a model for mammalian tubule function.Current opinion in nephrology and hypertension · 2019Review
- Luminescence-activated nucleotide cyclase regulates spatial and temporal cAMP synthesis.The Journal of biological chemistry · 2019Article
- Intracellular cAMP Sensor EPAC: Physiology, Pathophysiology, and Therapeutics Development.Physiological reviews · 2018Review
- Null EPAC mutants reveal a sequential order of versatile cAMP effects duringLearning & memory (Cold Spring Harbor, N.Y.) · 2017Article
- Cyclic nucleotide signaling is required during synaptic refinement at the Drosophila neuromuscular junction.Developmental neurobiology · 2017Article
- Bacillus bombysepticus α-Toxin Binding to G Protein-Coupled Receptor Kinase 2 Regulates cAMP/PKA Signaling Pathway to Induce Host Death.PLoS pathogens · 2016Article
- Use of the Ramsay Assay to Measure Fluid Secretion and Ion Flux Rates in the Drosophila melanogaster Malpighian Tubule.Journal of visualized experiments : JoVE · 2015Article
- Two inwardly rectifying potassium channels, Irk1 and Irk2, play redundant roles in Drosophila renal tubule function.American journal of physiology. Regulatory, integrative and comparative physiology · 2015Article
- Cardiac cAMP: production, hydrolysis, modulation and detection.Frontiers in pharmacology · 2015Review
- The genetically encoded tool set for investigating cAMP: more than the sum of its parts.Frontiers in pharmacology · 2015Review
- Activated astrocytes enhance the dopaminergic differentiation of stem cells and promote brain repair through bFGF.Nature communications · 2014Article
- Near-infrared light responsive synthetic c-di-GMP module for optogenetic applications.ACS synthetic biology · 2014Article
- Engineering adenylate cyclases regulated by near-infrared window light.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Cyclic AMP (cAMP) is a ubiquitous second messenger that regulates a variety of essential processes in diverse cell types, functioning via cAMP-dependent effectors such as protein kinase A (PKA) and/or exchange proteins directly activated by cAMP (EPAC). In an intact tissue it is difficult to separate the contribution of each cAMP effector in a particular cell type using genetic or pharmacological approaches alone. We, therefore, utilized optogenetics to overcome the difficulties associated with examining a multicellular tissue. The transgenic photoactive adenylyl cyclase bPAC can be activated to rapidly and reversibly generate cAMP pulses in a cell-type-specific manner. This optogenetic approach to cAMP manipulation was validated in vivo using GAL4-driven UAS-bPAC in a simple epithelium, the Drosophila renal (Malpighian) tubules. As bPAC was expressed under the control of cell-type-specific promoters, each cAMP signal could be directed to either the stellate or principal cells, the two major cell types of the Drosophila renal tubule. By combining the bPAC transgene with genetic and pharmacological manipulation of either PKA or EPAC it was possible to investigate the functional impact of PKA and EPAC independently of each other. The results of this investigation suggest that both PKA and EPAC are involved in cAMP sensing, but are engaged in very different downstream physiological functions in each cell type: PKA is necessary for basal secretion in principal cells only, and for stimulated fluid secretion in stellate cells only. By contrast, EPAC is important in stimulated fluid secretion in both cell types. We propose that such optogenetic control of cellular cAMP levels can be applied to other systems, for example the heart or the central nervous system, to investigate the physiological impact of cAMP-dependent signaling pathways with unprecedented precision.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.