ArticleEukaryotic cell2013
Evidence for recycling of invariant surface transmembrane domain proteins in African trypanosomes.
Article in Eukaryotic cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Cryo-EM structures of Trypanosoma brucei gambiense ISG65 with human complement C3 and C3b and their roles in alternative pathway restriction.Nature communications · 2023Article
- The role of invariant surface glycoprotein 75 in xenobiotic acquisition by African trypanosomes.Microbial cell (Graz, Austria) · 2023Article
- A multifaceted strategy to improve recombinant expression and structural characterisation of a Trypanosoma invariant surface protein.Scientific reports · 2022Article
- The History of Anti-Trypanosome Vaccine Development Shows That Highly Immunogenic and Exposed Pathogen-Derived Antigens Are Not Necessarily Good Target Candidates: Enolase and ISG75 as Examples.Pathogens (Basel, Switzerland) · 2021Article
- Late ESCRT machinery mediates the recycling and Rescue of Invariant Surface Glycoprotein 65 in Trypanosoma brucei.Cellular microbiology · 2020Article
- Establishment of a Standardized Vaccine Protocol for the Analysis of Protective Immune Responses During Experimental Trypanosome Infections in Mice.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Rab11 mediates selective recycling and endocytic trafficking in Trypanosoma brucei.Traffic (Copenhagen, Denmark) · 2018Article
- Serological tests for gambiense human African trypanosomiasis detect antibodies in cattle.Parasites & vectors · 2017Article
- The Trypanosome Exocyst: A Conserved Structure Revealing a New Role in Endocytosis.PLoS pathogens · 2017Article
- Modulation of the Surface Proteome through Multiple Ubiquitylation Pathways in African Trypanosomes.PLoS pathogens · 2015Article
- Receptor-mediated Endocytosis 8 Utilizes an N-terminal Phosphoinositide-binding Motif to Regulate Endosomal Clathrin Dynamics.The Journal of biological chemistry · 2015Article
- Rme-8 depletion perturbs Notch recycling and predisposes to pathogenic signaling.The Journal of cell biology · 2015Article
- Proteomic selection of immunodiagnostic antigens for Trypanosoma congolense.PLoS neglected tropical diseases · 2014Article
- Life and times: synthesis, trafficking, and evolution of VSG.Trends in parasitology · 2014Review
- RME-8 coordinates the activity of the WASH complex with the function of the retromer SNX dimer to control endosomal tubulation.Journal of cell science · 2014Article
- Protozoacidal Trojan-Horse: use of a ligand-lytic peptide for selective destruction of symbiotic protozoa within termite guts.PloS one · 2014Article
- AAK1-like: A putative pseudokinase with potential roles in cargo uptake in bloodstream form Trypanosoma brucei parasites.The Journal of eukaryotic microbiologyArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Intracellular trafficking is a vital component of both virulence mechanisms and drug interactions in Trypanosoma brucei, the causative agent of human African trypanosomiasis and n'agana of cattle. Both maintaining the surface proteome composition within a life stage and remodeling the composition when progressing between life stages are important features of immune evasion and development for trypanosomes. Our recent work implicates the abundant transmembrane invariant surface glycoproteins (ISGs) in the uptake of first-line therapeutic suramin, suggesting a potential therapeutic route into the cell. RME-8 is a mediator of recycling pathways in higher eukaryotes and is one of a small cohort of intracellular transport gene products upregulated in mammal-infective trypanosomes, suggesting a role in controlling the copy number of surface proteins in trypanosomes. Here we investigate RME-8 function and its contribution to intracellular trafficking and stability of ISGs. RME-8 is a highly conserved protein and is broadly distributed across multiple endocytic compartments. By knockdown we find that RME-8 is essential and mediates delivery of endocytic probes to late endosomal compartments. Further, we find ISG accumulation within endosomes, but that RME-8 knockdown also increases ISG turnover; combined with previous data, this suggests that it is most probable that ISGs are recycled, and that RME-8 is required to support recycling.
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Registered trials
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