ArticleJournal of bacteriology2013
The GTPase activity of FlhF is dispensable for flagellar localization, but not motility, in Pseudomonas aeruginosa.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- FlhF interacts with TonB2 to defend against oxidative stress in Campylobacter jejuni.BMC microbiology · 2026Article
- Article
- Where and how many: evolutionary diversification of a molecular switch regulating flagellar patterns.Journal of bacteriology · 2026Review
- Role of FlhF and its domains in the assembly of a polar flagellum inJournal of bacteriology · 2025Article
- Article
- Bacterial cell surface characterization by phage display coupled to high-throughput sequencing.Nature communications · 2024Article
- Silver Ions Inhibit Bacterial Movement and Stall Flagellar Motor.International journal of molecular sciences · 2023Article
- Comparative transcriptome combined with morphophysiological analyses revealed the molecular mechanism underlyingVirulence · 2022Article
- Indonesian MangroveMolecules (Basel, Switzerland) · 2022Article
- Review
- Interdependent Polar Localization of FlhF and FlhG and Their Importance for Flagellum Formation ofFrontiers in microbiology · 2021Article
- FlhF regulates the number and configuration of periplasmic flagella in Borrelia burgdorferi.Molecular microbiology · 2020Article
- Assembly mechanism of a supramolecular MS-ring complex to initiate bacterial flagellar biogenesis inJournal of bacteriology · 2020Article
- Investigating the Role of FlhF Identifies Novel Interactions With Genes Involved in Flagellar Synthesis inFrontiers in microbiology · 2020Article
- GTP-Dependent FlhF Homodimer Supports Secretion of a Hemolysin inFrontiers in microbiology · 2020Article
- Modulation of flagellar rotation in surface-attached bacteria: A pathway for rapid surface-sensing after flagellar attachment.PLoS pathogens · 2019Article
- Functional Regulators of Bacterial Flagella.Annual review of microbiology · 2019Review
- Insights into the impact of flhF inactivation on Campylobacter jejuni colonization of chick and mice gut.BMC microbiology · 2018Article
- Biochemical analysis of GTPase FlhF which controls the number and position of flagellar formation in marine Vibrio.Scientific reports · 2018Article
- Analysis of the GTPase motif of FlhF in the control of the number and location of polar flagella inBiophysics and physicobiology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The opportunistic human pathogen Pseudomonas aeruginosa uses two surface organelles, flagella and pili, for motility and adhesion in biotic and abiotic environments. Polar flagellar placement and number are influenced by FlhF, which is a signal recognition particle (SRP)-type GTPase. The FlhF proteins of Bacillus subtilis and Campylobacter jejuni were recently shown to have GTPase activity. However, the phenotypes associated with flhF deletion and/or mutation differ between these organisms and P. aeruginosa, making it difficult to generalize a role for FlhF in pseudomonads. In this study, we confirmed that FlhF of P. aeruginosa binds and hydrolyzes GTP. We mutated FlhF residues that we predicted would alter nucleotide binding and hydrolysis and determined the effects of these mutations on FlhF enzymatic activity, protein dimerization, and bacterial motility. Both hydrolytically active and inactive FlhF point mutants restored polar flagellar assembly, as seen for wild-type FlhF. However, differential effects on flagellar function were observed in single-cell assays of swimming motility and flagellar rotation. These findings indicate that FlhF function is influenced by its nucleotide binding and hydrolytic activities and demonstrate that FlhF affects P. aeruginosa flagellar function as well as assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.