ArticleJournal of bacteriology2013
Requirements for in vitro germination of Paenibacillus larvae spores.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 50 citations in OpenAlex.
- Comparative genomics and phenotypic divergence of ERIC I and ERIC II genotypes of Paenibacillus larvae, the causative agent of American Foulbrood disease.PLoS pathogens · 2026Article
- In Vivo and In Vitro Evaluation of Probiotic and Postbiotic Strategies Against Foulbrood in Honeybees.Probiotics and antimicrobial proteins · 2026Article
- Development of an algae extract-based culture medium forMethodsX · 2025Article
- Article
- Bacteriophage resistance evolution in a honey bee pathogen.Microbiology (Reading, England) · 2025Article
- Differential impact ofHeliyon · 2024Article
- Viral adaptations to alternative hosts in the honey bee pathogenbioRxiv : the preprint server for biology · 2024Article
- Bacterial Strains Isolated from Stingless Bee Workers Inhibit the Growth of Apis mellifera Pathogens.Current microbiology · 2024Article
- Oxytetracycline-resistantJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023Article
- Probiotics and in-hive fermentation as a source of beneficial microbes to support the gut microbial health of honey bees.Journal of insect science (Online) · 2023Review
- Use of Dicranum polysetum extract against Paenibacillus larvae causing American Foulbrood under in vivo and in vitro conditions.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Beneficial bacteria as biocontrol agents for American foulbrood disease in honey bees (Apis mellifera).Journal of insect science (Online) · 2023Review
- Establishment of apiary-level risk of American foulbrood through the detection of Paenibacillus larvae spores in pooled, extracted honey in Saskatchewan.Scientific reports · 2022Article
- Comparison of individual hive and apiary-level sample types for spores of Paenibacillus larvae in Saskatchewan honey bee operations.PloS one · 2022Article
- Fructose and Trehalose Selectively Enhance In Vitro Sporulation ofMicroorganisms · 2021Article
- Phages Bind to Vegetative and Spore Forms ofFrontiers in microbiology · 2021Article
- Novel probiotic approach to counter Paenibacillus larvae infection in honey bees.The ISME journal · 2020Article
- Article
- Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model.Scientific reports · 2019Article
- 10-HDA, A Major Fatty Acid of Royal Jelly, Exhibits pH Dependent Growth-Inhibitory Activity Against Different Strains ofMolecules (Basel, Switzerland) · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Paenibacillus larvae is the causative agent of American foulbrood (AFB), a disease affecting honey bee larvae. First- and second-instar larvae become infected when they ingest food contaminated with P. larvae spores. The spores then germinate into vegetative cells that proliferate in the midgut of the honey bee. Although AFB affects honey bees only in the larval stage, P. larvae spores can be distributed throughout the hive. Because spore germination is critical for AFB establishment, we analyzed the requirements for P. larvae spore germination in vitro. We found that P. larvae spores germinated only in response to l-tyrosine plus uric acid under physiologic pH and temperature conditions. This suggests that the simultaneous presence of these signals is necessary for spore germination in vivo. Furthermore, the germination profiles of environmentally derived spores were identical to those of spores from a biochemically typed strain. Because l-tyrosine and uric acid are the only required germinants in vitro, we screened amino acid and purine analogs for their ability to act as antagonists of P. larvae spore germination. Indole and phenol, the side chains of tyrosine and tryptophan, strongly inhibited P. larvae spore germination. Methylation of the N-1 (but not the C-3) position of indole eliminated its ability to inhibit germination. Identification of the activators and inhibitors of P. larvae spore germination provides a basis for developing new tools to control AFB.
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