ArticleRNA (New York, N.Y.)2013
Transcriptome-wide identification of A > I RNA editing sites by inosine specific cleavage.
Article in RNA (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 81 citations in OpenAlex.
- ENDONUCLEASE V: FROM TRANSCRIPTOME REGULATOR TO CHEMICAL BIOLOGY TOOL.ChemistryEurope · 2026Article
- Advances in Quantitative Techniques for Mapping RNA Modifications.Life (Basel, Switzerland) · 2025Review
- ADAR1 editing is necessary for only a small subset of cytosolic dsRNAs to evade MDA5-mediated autoimmunity.Nature genetics · 2025Article
- A-to-I mRNA editing recodes hundreds of genes in dozens of species and produces endogenous protein isoforms in bacteria.Nucleic acids research · 2025Article
- A-to-I mRNA editing in bacteria can affect protein sequence, disulfide bond formation, and function.Nucleic acids research · 2025Article
- Coding-Sequence Evolution Does Not Explain Divergence in Petal Anthocyanin Pigmentation Between Mimulus luteus Var luteus and M. l. variegatus.Evolution & development · 2025Article
- High-throughput detection of RNA modifications at single base resolution.FEBS letters · 2025Review
- Antizyme inhibitor family: biological and translational research implications.Cell communication and signaling : CCS · 2024Review
- Deep transcriptome profiling reveals limited conservation of A-to-I RNA editing in Xenopus.BMC biology · 2023Article
- Transcriptome-wide profiling of A-to-I RNA editing by Slic-seq.Nucleic acids research · 2023Article
- Regulation of the epigenome through RNA modifications.Chromosoma · 2023Review
- Absolute quantification of single-base mNature biotechnology · 2023Article
- Exploring the epitranscriptome by native RNA sequencing.RNA (New York, N.Y.) · 2022Article
- 8-azaadenosine and 8-chloroadenosine are not selective inhibitors of ADAR.Cancer research communications · 2021Article
- Recent technical advances in the study of nucleic acid modifications.Molecular cell · 2021Review
- Long Non-Coding RNA Epigenetics.International journal of molecular sciences · 2021Review
- A mark of disease: how mRNA modifications shape genetic and acquired pathologies.RNA (New York, N.Y.) · 2021Review
- A Census and Categorization Method of Epitranscriptomic Marks.International journal of molecular sciences · 2020Review
- Epitranscriptomic marks: Emerging modulators of RNA virus gene expression.Wiley interdisciplinary reviews. RNA · 2020Review
- Broadly applicable oligonucleotide mass spectrometry for the analysis of RNA writers and erasers in vitro.Nucleic acids research · 2020Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adenosine to inosine (A > I) RNA editing, which is catalyzed by the ADAR family of proteins, is one of the fundamental mechanisms by which transcriptomic diversity is generated. Indeed, a number of genome-wide analyses have shown that A > I editing is not limited to a few mRNAs, as originally thought, but occurs widely across the transcriptome, especially in the brain. Importantly, there is increasing evidence that A > I editing is essential for animal development and nervous system function. To more efficiently characterize the complete catalog of ADAR events in the mammalian transcriptome we developed a high-throughput protocol to identify A > I editing sites, which exploits the capacity of glyoxal to protect guanosine, but not inosine, from RNAse T1 treatment, thus facilitating extraction of RNA fragments with inosine bases at their termini for high-throughput sequencing. Using this method we identified 665 editing sites in mouse brain RNA, including most known sites and suite of novel sites that include nonsynonymous changes to protein-coding genes, hyperediting of genes known to regulate p53, and alterations to non-protein-coding RNAs. This method is applicable to any biological system for the de novo discovery of A > I editing sites, and avoids the complicated informatic and practical issues associated with editing site identification using traditional RNA sequencing data. This approach has the potential to substantially increase our understanding of the extent and function of RNA editing, and thereby to shed light on the role of transcriptional plasticity in evolution, development, and cognition.
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Registered trials
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