ArticlePlant molecular biology2013
Identification of a canonical SCF(SLF) complex involved in S-RNase-based self-incompatibility of Pyrus (Rosaceae).
Article in Plant molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 45 citations in OpenAlex.
- SDJ, a pollen-expressed type III J-protein in Prunus, directly binds S-RNase and promotes recruitment of SLFL6 to an S-RNase-associated complex.Plant reproduction · 2026Article
- Pan-genome cloning and expression analysis of S-RNase homologous gene in SI and SC of different tomato species.Scientific reports · 2026Article
- S-RNase-based self-incompatibility in angiosperms: Degradation, condensation, and evolution.Plant physiology · 2025Review
- Molecular mechanisms and genetic regulation of self-incompatibility in flowering plants: implications for crop improvement and evolutionary biology.Plant molecular biology · 2025Review
- Rapid detection of RNase-based self-incompatibility in Lysimachia monelli (Primulaceae).American journal of botany · 2025Article
- The Identification and Analysis of the Self-Incompatibility Pollen Determinant FactorPlants (Basel, Switzerland) · 2024Article
- Transcriptome Analysis of the Late-Acting Self-Incompatibility Associated with RNase T2 Family inPlants (Basel, Switzerland) · 2023Article
- Analyses of Cullin1 homologs reveal functional redundancy in S-RNase-based self-incompatibility and evolutionary relationships in eudicots.The Plant cell · 2023Article
- Genome-wide identification of members of thePhysiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- The Core Jasmonic Acid-Signalling ModuleCurrent issues in molecular biology · 2022Article
- Genome-Wide Analysis of theInternational journal of molecular sciences · 2022Article
- Primary restriction of S-RNase cytotoxicity by a stepwise ubiquitination and degradation pathway in Petunia hybrida.The New phytologist · 2021Article
- In-depth Understanding ofInternational journal of molecular sciences · 2020Article
- Article
- Identification of a Novel SBP1-Containing SCFFrontiers in genetics · 2019Article
- Finding a Compatible Partner: Self-Incompatibility in European Pear (Frontiers in plant science · 2019Review
- CRISPR/Cas9-mediated knockout of PiSSK1 reveals essential role of S-locus F-box protein-containing SCF complexes in recognition of non-self S-RNases during cross-compatible pollination in self-incompatible Petunia inflata.Plant reproduction · 2018Article
- Characterization and comparative expression analysis of CUL1 genes in rice.Genes & genomics · 2018Article
- Lack of S-RNase-Based Gametophytic Self-Incompatibility in Orchids Suggests That This System Evolved after the Monocot-Eudicot Split.Frontiers in plant science · 2017Article
- Recognition of a wide-range of S-RNases by S locus F-box like 2, a general-inhibitor candidate in the Prunus-specific S-RNase-based self-incompatibility system.Plant molecular biology · 2016Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
S-RNase-based self-incompatibility (SI) is an intraspecific reproductive barrier to prevent self-fertilization found in many species of the Solanaceae, Plantaginaceae and Rosaceae. In this system, S-RNase and SLF/SFB (S-locus F-box) genes have been shown to control the pistil and pollen SI specificity, respectively. Recent studies have shown that the SLF functions as a substrate receptor of a SCF (Skp1/Cullin1/F-box)-type E3 ubiquitin ligase complex to target S-RNases in Solanaceae and Plantaginaceae, but its role in Rosaceae remains largely undefined. Here we report the identification of two pollen-specific SLF-interacting Skp1-like (SSK) proteins, PbSSK1 and PbSSK2, in Pyrus bretschneideri from the tribe Pyreae of Rosaceae. Both yeast two-hybrid and pull-down assays demonstrated that they could connect PbSLFs to PbCUL1 to form a putative canonical SCF(SLF) (SSK/CUL1/SLF) complex in Pyrus. Furthermore, pull-down assays showed that the SSK proteins could bind SLF and CUL1 in a cross-species manner between Pyrus and Petunia. Additionally, phylogenetic analysis revealed that the SSK-like proteins from Solanaceae, Plantaginaceae and Rosaceae form a monoclade group, hinting their shared evolutionary origin. Taken together, with the recent identification of a canonical SCF(SFB) complex in Prunus of the tribe Amygdaleae of Rosaceae, our results show that a conserved canonical SCF(SLF/SFB) complex is present in Solanaceae, Plantaginaceae and Rosaceae, implying that S-RNase-based self-incompatibility shares a similar molecular and biochemical mechanism.
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