ArticleJournal of assisted reproduction and genetics2013
Quality and functionality of human ovarian tissue after cryopreservation using an original slow freezing procedure.
Article in Journal of assisted reproduction and genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Comparison of the quality of ovarian tissue cryopreservation by conventional slow cryopreservation and vitrification-a systematic review and meta-analysis.Journal of ovarian research · 2025Pooled it
- Treatment with trypLE before freezing improves thawing integrity and functionality of sheep ovarian tissue.Journal of ovarian research · 2025Article
- Optimizing the Ovarian Tissue Cryopreservation in the 'Oncofertility' Institutional Program at an Italian National Cancer Institute.Healthcare (Basel, Switzerland) · 2023Article
- Nanowarming improves survival of vitrified ovarian tissue and follicular development in a sheep model.Heliyon · 2023Article
- RNA Transcripts in Human Ovarian Cells: Two-Time Cryopreservation Does Not Affect Developmental Potential.International journal of molecular sciences · 2023Article
- Glutamine and norepinephrine in follicular fluid synergistically enhance the antioxidant capacity of human granulosa cells and the outcome of IVF-ET.Scientific reports · 2022Article
- DynamicFrontiers in endocrinology · 2022Article
- Fresh and cryopreserved ovarian tissue from deceased young donors yields viable follicles.F&S science · 2021Article
- Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles.Scientific reports · 2020Article
- Slow Freezing Versus Vitrification of Mouse Ovaries: from Ex Vivo Analyses to Successful Pregnancies after Auto-Transplantation.Scientific reports · 2019Article
- Article
- Comparison of the Oocyte Quality Derived from Two-Dimensional Follicle Culture Methods and Developmental Competence ofBioMed research international · 2018Article
- Validation of an automated technique for ovarian cortex dissociation: isolation of viable ovarian cells and their qualification by multicolor flow cytometry.Journal of ovarian research · 2017Article
- Clinically applied procedures for human ovarian tissue cryopreservation result in different levels of efficacy and efficiency.Journal of assisted reproduction and genetics · 2016Article
- Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) pre-exposure ensures follicle integrity during in vitro culture of ovarian tissue but not during cryopreservation in the domestic cat model.Journal of assisted reproduction and genetics · 2016Article
- Supplementation of transport and freezing media with anti-apoptotic drugs improves ovarian cortex survival.Journal of ovarian research · 2016Article
- Vitrification of human ovarian tissue: a practical and relevant alternative to slow freezing.Reproductive biology and endocrinology : RB&E · 2015Article
- Isoform 165 of vascular endothelial growth factor in collagen matrix improves ovine cryopreserved ovarian tissue revascularisation after xenotransplantation in mice.Reproductive biology and endocrinology : RB&E · 2015Article
- Efficacy of ovarian tissue cryopreservation in a major European center.Journal of assisted reproduction and genetics · 2014Article
- Ovarian follicle culture: advances and challenges for human and nonhuman primates.Fertility and sterility · 2013Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo evaluate the efficiency of an original slow freezing protocol on the quality and function of human ovarian cortex.
methodsHuman ovarian tissues were cryopreserved using a freezing medium supplemented with propanediol and raffinose as cryoprotectants and antioxidants (L-glutamine, taurine). Samples were then frozen using a faster cooling rate than the usual one. Viability and morphology of follicles, DNA fragmentation in follicles and stroma as well as histology of the vascular endothelium were analyzed before and after freezing/thawing. Moreover, a functional analysis was performed based on the evaluation of follicular growth and development in thawed ovarian tissues that were cultured in vitro.
resultsOur freezing/thawing protocol allows preservation of a high proportion of viable follicles and the preservation of the different follicle developmental stages (p>0.05 versus fresh control). 70.5 ± 5.2 % of follicles retained an intact morphology after cryopreservation (p=0.04). Stroma cells but not follicles exhibited a slight increase of DNA fragmentation after thawing (p<0.05). Microvessel endothelium within thawed tissues appeared to be preserved. Granulosa cells showed signs of proliferation in follicles cultured for 12 days. Secretion of 17β-oestradiol significantly increased during in vitro culture.
conclusionsThis protocol leads to good preservation of ovarian integrity and functionality post-thawing and thus appears as a suitable technique of ovarian tissue cryopreservation in clinical settings. Further research could be extended to optimize conditions of in vitro culture.
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