ArticleCurrent microbiology2013
Assessment of cagE and babA mRNA expression during morphological conversion of Helicobacter pylori from spiral to coccoid.
Article in Current microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Seven-day quintuple regimen as a rescue therapy for Helicobacter pylori eradication.World journal of gastroenterology · 2015Trial
- Determination of GenesThe Yale journal of biology and medicine · 2025Article
- Co-culture of Helicobacter pylori with oral microorganisms in human saliva.Clinical oral investigations · 2025Article
- Viable but non-cultivable state in oral microbiota: a critical review of an underexplored microbial survival strategy.Frontiers in cellular and infection microbiology · 2025Review
- Study of the relationships among known virulence genes, coccoid transformation and cytotoxicity ofVirulence · 2024Article
- [The Recurrence ofThe Korean journal of helicobacter and upper gastrointestinal research · 2023Article
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- Research progress in theZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2021Article
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- Helicobacter pylori metabolites exacerbate gastritis through C-type lectin receptors.The Journal of experimental medicine · 2021Article
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- Clinical and laboratory importance of detectingPrzeglad gastroenterologiczny · 2020Review
- Helicobacter pylori recrudescence and its influencing factors.Journal of cellular and molecular medicine · 2019Review
- Potential Role of Biofilm Formation in the Development of Digestive Tract Cancer With Special Reference toFrontiers in microbiology · 2019Article
- Overexpression of spoT gene in coccoid forms of clinical Helicobacter pylori isolates.Folia microbiologica · 2018Article
- Significance of dormant forms ofWorld journal of gastroenterology · 2017Review
- Understanding the dimorphic lifestyles of human gastric pathogen Helicobacter pylori using the SWATH-based proteomics approach.Scientific reports · 2016Article
- Geranylgeranylacetone selectively binds to the HSP70 of Helicobacter pylori and alters its coccoid morphology.Scientific reports · 2015Article
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Authors and funding
8 authors.
Funding
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Abstract
Helicobacter pylori (H. Pylori) is an actively dividing spiral bacterium that changes to coccoid morphology under stressful environments. The infectivity of the coccoids is still controversial. The aim of this study was to determine the viability and expression of two important virulence genes (babA and cagE), in antibiotic-induced coccoid forms. Three strains of H. pylori, the standard 26695 and two clinical isolates (p1, p2) were converted to coccoid form by amoxicillin. Coccoids were identified according to Gram-staining and microscopic morphology. The viability of the cells was analyzed by flow cytometry. The expression of cagE and babA in coccoid forms were evaluated and compared to the spirals by quantitative PCR assay. The coccoid forms were developed after 72 h exposure of H. pylori to ½ MIC of amoxicillin, and the conversion form was completed (100 %) at 144 h in all of three isolates. Flow cytometry analyses showed that the majority of the induced coccoids (90-99.9 %) were viable. Expression of cagE and babA was seen in coccoids; however, in lower rate (cagE, ~3-fold and babA, ~10-fold) than these in spiral forms. Coccoid forms of two clinical isolates significantly expressed higher rate of cagE and babA than standard 26695 strain (P = 0.01). These results suggest that the induced coccoid form of H. pylori is not a passive entity but can actively infect the human by expression of the virulence genes for long time in stomach and probably play a role in chronic and severe disease.
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