ArticleOptics express2012
Three dimensional HiLo-based structured illumination for a digital scanned laser sheet microscopy (DSLM) in thick tissue imaging.
Article in Optics express, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Insight into Single-Molecule Imaging Techniques for the Study of Prokaryotic Genome Maintenance.Chemical & biomedical imaging · 2024Review
- Image improvement of temporal focusing multiphoton microscopy via superior spatial modulation excitation and Hilbert-Huang transform decomposition.Scientific reports · 2022Article
- Strain maps characterize the symmetry of convergence and extension patterns during zebrafish gastrulation.Scientific reports · 2021Article
- Temporal focusing multiphoton microscopy with optimized parallel multiline scanning for fast biotissue imaging.Journal of biomedical optics · 2021Article
- Learning to synthesize: robust phase retrieval at low photon counts.Light, science & applications · 2020Article
- Improved contrast in inverted selective plane illumination microscopy of thick tissues using confocal detection and structured illumination.Biomedical optics express · 2017Article
- Three-dimensional image cytometer based on widefield structured light microscopy and high-speed remote depth scanning.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015Article
- Depth resolved hyperspectral imaging spectrometer based on structured light illumination and Fourier transform interferometry.Biomedical optics express · 2014Article
- Talbot holographic illumination nonscanning (THIN) fluorescence microscopy.Laser & photonics reviews · 2014Article
- Improvement of axial resolution and contrast in temporally focused widefield two-photon microscopy with structured light illumination.Biomedical optics express · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Laser sheet based microscopy has become widely accepted as an effective active illumination method for real time three-dimensional (3D) imaging of biological tissue samples. The light sheet geometry, where the camera is oriented perpendicular to the sheet itself, provides an effective method of eliminating some of the scattered light and minimizing the sample exposure to radiation. However, residual background noise still remains, limiting the contrast and visibility of potentially interesting features in the samples. In this article, we investigate additional structuring of the illumination for improved background rejection, and propose a new technique, "3D HiLo" where we combine two HiLo images processed from orthogonal directions to improve the condition of the 3D reconstruction. We present a comparative study of conventional structured illumination based demodulation methods, namely 3Phase and HiLo with a newly implemented 3D HiLo approach and demonstrate that the latter yields superior signal-to-background ratio in both lateral and axial dimensions, while simultaneously suppressing image processing artifacts.
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Registered trials
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