ArticleNucleic acids research2013
Solution conformations of early intermediates in Mos1 transposition.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- mariner elements as a model for analyzing the stress response and somatic mobilization activity of transposable elements.Heredity · 2025Review
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- Production of perdeuterated fucose from glyco-engineered bacteria.Glycobiology · 2021Article
- Matchout deuterium labelling of proteins for small-angle neutron scattering studies using prokaryotic and eukaryotic expression systems and high cell-density cultures.European biophysics journal : EBJ · 2017Article
- Regulated complex assembly safeguards the fidelity of Sleeping Beauty transposition.Nucleic acids research · 2017Article
- Structural Determinants of Sleeping Beauty Transposase Activity.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- Article
- Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering.Nature communications · 2016Article
- Evolutionary history of the Azteca-like mariner transposons and their host ants.Die Naturwissenschaften · 2015Article
- The pentameric nucleoplasmin fold is present in Drosophila FKBP39 and a large number of chromatin-related proteins.Journal of molecular biology · 2015Article
- Structural Basis for the Inverted Repeat Preferences of mariner Transposases.The Journal of biological chemistry · 2015Article
- Mechanisms of DNA Transposition.Microbiology spectrum · 2015Review
- Biosynthetic preparation of selectively deuterated phosphatidylcholine in genetically modified Escherichia coli.Applied microbiology and biotechnology · 2015Article
- Article
- Biochemical characterization and comparison of two closely related active mariner transposases.Biochemistry · 2014Article
- Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis.Nucleic acids research · 2014Article
- Target capture during Mos1 transposition.The Journal of biological chemistry · 2014Article
- Article
- One to rule them all: A highly conserved motif in mariner transposase controls multiple steps of transposition.Mobile genetic elements · 2014Article
- cAMP protein kinase phosphorylates the Mos1 transposase and regulates its activity: evidences from mass spectrometry and biochemical analyses.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
DNA transposases facilitate genome rearrangements by moving DNA transposons around and between genomes by a cut-and-paste mechanism. DNA transposition proceeds in an ordered series of nucleoprotein complexes that coordinate pairing and cleavage of the transposon ends and integration of the cleaved ends at a new genomic site. Transposition is initiated by transposase recognition of the inverted repeat sequences marking each transposon end. Using a combination of solution scattering and biochemical techniques, we have determined the solution conformations and stoichiometries of DNA-free Mos1 transposase and of the transposase bound to a single transposon end. We show that Mos1 transposase is an elongated homodimer in the absence of DNA and that the N-terminal 55 residues, containing the first helix-turn-helix motif, are required for dimerization. This arrangement is remarkably different from the compact, crossed architecture of the dimer in the Mos1 paired-end complex (PEC). The transposase remains elongated when bound to a single-transposon end in a pre-cleavage complex, and the DNA is bound predominantly to one transposase monomer. We propose that a conformational change in the single-end complex, involving rotation of one half of the transposase along with binding of a second transposon end, could facilitate PEC assembly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.