ArticleNucleic acids research2013
Coordinated induction of multi-gene pathways in Saccharomyces cerevisiae.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
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- Pathway engineering of Saccharomyces cerevisiae for efficient lycopene production.Bioprocess and biosystems engineering · 2021Article
- Advances in biotechnological production of santalenes and santalols.Chinese herbal medicines · 2021Review
- Metabolic Engineering of Non-carotenoid-Producing YeastFrontiers in microbiology · 2021Article
- An artificial chromosome ylAC enables efficient assembly of multiple genes in Yarrowia lipolytica for biomanufacturing.Communications biology · 2020Article
- Biotechnological production of betulinic acid and derivatives and their applications.Applied microbiology and biotechnology · 2020Review
- A universal gene expression system for fungi.Nucleic acids research · 2018Article
- Pulsatile inputs achieve tunable attenuation of gene expression variability and graded multi-gene regulation.Nature communications · 2018Article
- Synthetic Transcription Amplifier System for Orthogonal Control of Gene Expression in Saccharomyces cerevisiae.PloS one · 2016Article
- Engineered biosynthesis of natural products in heterologous hosts.Chemical Society reviews · 2015Review
- The development and characterization of synthetic minimal yeast promoters.Nature communications · 2015Article
- Exploiting Issatchenkia orientalis SD108 for succinic acid production.Microbial cell factories · 2014Article
- Engineering microbial cells for the biosynthesis of natural compounds of pharmaceutical significance.BioMed research international · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Bacterial operons are nature's tool for regulating and coordinating multi-gene expression in prokaryotes. They are also a gene architecture commonly used in the biosynthesis of many pharmaceutically important compounds and industrially useful chemicals. Despite being an important eukaryotic production host, Saccharomyces cerevisiae has never had such gene architecture. Here, we report the development of a system to assemble and regulate a multi-gene pathway in S. cerevisiae. Full pathways can be constructed using pre-made parts from a plasmid toolbox. Subsequently, through the use of a yeast strain containing a stably integrated gene switch, the assembled pathway can be regulated using a readily available and inexpensive compound-estradiol-with extremely high sensitivity (10 nM). To demonstrate the use of the system, we assembled the five-gene zeaxanthin biosynthetic pathway in a single step and showed the ligand-dependent coordinated expression of all five genes as well as the tightly regulated production of zeaxanthin. Compared with a previously reported constitutive zeaxanthin pathway, our inducible pathway was shown to have 50-fold higher production level.
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