ArticleThe Journal of biological chemistry2013
Interactions of Anopheles gambiae odorant-binding proteins with a human-derived repellent: implications for the mode of action of n,n-diethyl-3-methylbenzamide (DEET).
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- A Safer Alternative Bio-Repellent: Targeting Mosquito Odorant-Binding Proteins with Catnip-Derived Nepetalactones fromInternational journal of molecular sciences · 2026Article
- In vitro larvicidal activity of selected azabenzimidazole and diarylquinoline derivatives against Anopheles gambiae and in silico mechanistic analysis.Molecular diversity · 2026Article
- AgamOBP1-Directed Discovery of Repellents to Control the Spread of Mosquito-Borne Diseases.ChemMedChem · 2025Article
- Lemongrass essential oil and DEET inhibit attractant detection in infected and non-infectedCurrent research in insect science · 2024Article
- Omics and Remote Homology Integration to Decipher Protein Functionality.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Molecular insights on ar-turmerone as a structural, functional and pharmacophoric analogue of synthetic mosquito repellent DEET by comprehensive computational assessment.Scientific reports · 2022Article
- Host-Guest Inclusion Complexes of Natural Products and Nanosystems: Applications in the Development of Repellents.Molecules (Basel, Switzerland) · 2022Review
- Article
- The Olfactory Chemosensation of Hematophagous Hemipteran Insects.Frontiers in physiology · 2021Review
- Olfaction in Anopheles mosquitoes.Chemical senses · 2021Review
- Aedes aegypti Odorant Binding Protein 22 selectively binds fatty acids through a conformational change in its C-terminal tail.Scientific reports · 2020Article
- Molecular characteristics of odorant-binding protein 1 in Anopheles maculipennis.Malaria journal · 2020Article
- Article
- Biosensors to Monitor Water Quality Utilizing Insect Odorant-Binding Proteins as Detector Elements.Biosensors · 2019Article
- Sex and age modulate antennal chemosensory-related genes linked to the onset of host seeking in the yellow-fever mosquito, Aedes aegypti.Scientific reports · 2019Article
- New insights on repellent recognition by Anopheles gambiae odorant-binding protein 1.PloS one · 2018Article
- Structural Transformation Detection Contributes to Screening of Behaviorally Active Compounds: Dynamic Binding Process Analysis of DhelOBP21 from Dastarcus helophoroides.Journal of chemical ecology · 2017Article
- Deciphering the olfactory repertoire of the tiger mosquito Aedes albopictus.BMC genomics · 2017Article
- The crystal structure of the AgamOBP1•Icaridin complex reveals alternative binding modes and stereo-selective repellent recognition.Cellular and molecular life sciences : CMLS · 2017Article
- Binding of a fluorescence reporter and a ligand to an odorant-binding protein of the yellow fever mosquito, Aedes aegypti.F1000Research · 2014Article
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Authors and funding
5 authors.
Funding
Abstract
The Anopheles gambiae mosquito, which is the vector for Plasmodium falciparum malaria, uses a series of olfactory cues emanating from human sweat to select humans as their source for a blood meal. Perception of these odors within the mosquito olfactory system involves the interplay of odorant-binding proteins (OBPs) and odorant receptors and disrupting the normal responses to those odorants that guide mosquito-human interactions represents an attractive approach to prevent the transmission of malaria. Previously, it has been shown that DEET targets multiple components of the olfactory system, including OBPs and odorant receptors. Here, we present the crystal structure of A. gambiae OBP1 (OBP1) in the complex it forms with a natural repellent 6-methyl-5-heptene-2-one (6-MH). We find that 6-MH binds to OBP1 at exactly the same site as DEET. However, key interactions with a highly conserved water molecule that are proposed to be important for DEET binding are not involved in binding of 6-MH. We show that 6-MH and DEET can compete for the binding of attractive odorants and in doing so disrupt the interaction that OBP1 makes with OBP4. We further show that 6-MH and DEET can bind simultaneously to OBPs with other ligands. These results suggest that the successful discovery of novel reagents targeting OBP function requires knowledge about the specific mechanism of binding to the OBP rather than their binding affinity.
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