ArticleChemistry & biology2012
Characterization of a fungal thioesterase having Claisen cyclase and deacetylase activities in melanin biosynthesis.
Article in Chemistry & biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 60 citations in OpenAlex.
- A hybrid assembly mechanism employs nonreducing polyketide synthase-like logic for partially reduced polyketide formation.Marine life science & technology · 2026Article
- Biosynthesis of the Paecilomyces marquandii conidial pigment saintopin.Fungal biology and biotechnology · 2025Article
- CRISPR/Cas9-assisted gene editing reveals that EgPKS, a polyketide synthase, is required for the biosynthesis of preussomerins in Edenia gomezpompae SV2.World journal of microbiology & biotechnology · 2025Article
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- The divergence of DHN-derived melanin pathways in Metarhizium robertsii.World journal of microbiology & biotechnology · 2024Article
- Polyketide Trimming Shapes Dihydroxynaphthalene-Melanin and Anthraquinone Pigments.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Efficient gene editing in the slow-growing, non-sporulating, melanized, endophytic fungus Berkleasmium sp. Dzf12 using a CRISPR/Cas9 system.World journal of microbiology & biotechnology · 2024Article
- Combinatorial biosynthesis for the engineering of novel fungal natural products.Communications chemistry · 2024Review
- The Ketosynthase Domain Controls Chain Length in Mushroom Oligocyclic Polyketide Synthases.Chembiochem : a European journal of chemical biology · 2023Article
- N-terminal lid swapping contributes to the substrate specificity and activity of thermophilic lipase TrLipE.Frontiers in microbiology · 2023Article
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- Article
- Sea Urchin Polyketide Synthase SpPks1 Produces the Naphthalene Precursor to Echinoderm Pigments.Journal of the American Chemical Society · 2022Article
- Comparative Genomics Reveals a Single Nucleotide Deletion inFrontiers in fungal biology · 2022Article
- Biosynthesis and biological function of secondary metabolites of the rice blast fungus Pyricularia oryzae.Journal of industrial microbiology & biotechnology · 2021Review
- Biosynthetic Cyclization Catalysts for the Assembly of Peptide and Polyketide Natural Products.ChemCatChem · 2021Article
- Secondary Metabolites of the Rice Blast FungusInternational journal of molecular sciences · 2020Review
- Structural Basis for Enzymatic Off-Loading of Hybrid Polyketides by Dieckmann Condensation.ACS chemical biology · 2020Article
- Function of pathway specific regulators in theRSC advances · 2019Article
- Dalmanol biosyntheses require coupling of two separate polyketide gene clusters.Chemical science · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Melanins are a broad class of darkly pigmented macromolecules formed by oxidative polymerization of phenolic monomers. In fungi, melanins are known virulence factors that contribute to pathogenicity. Their biosynthesis generally involves polymerization of 1,8-dihydroxynaphthalene via a 1,3,6,8-tetrahydroxynaphthalene (THN) precursor assembled by multidomain, nonreducing polyketide synthases. Convergent routes to THN have evolved in fungi. Parallel heptaketide and hexaketide pathways exist that utilize conventional C-terminal thioesterase/Claisen cyclase domains and separate side-chain deacylases. Here, in vitro characterization of Pks1 from Colletotrichum lagenarium establishes a true THN synthase with a bifunctional thioesterase (TE) catalyzing both cyclization and deacetylation of an enzyme-bound hexaketide substrate. Chimeric TE domains were generated by swapping lid regions of active sites between classes of melanin TEs to gain insight into this unprecedented catalysis of carbon-carbon bond making and breaking by an α/β-hydrolase fold enzyme.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.