ArticleBiomaterials2013
Melittin-grafted HPMA-oligolysine based copolymers for gene delivery.
Article in Biomaterials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Intrathecal drug delivery in the era of nanomedicine.Advanced drug delivery reviews · 2020Pooled it
- The reduced-charge melittin analogue MelP5 improves the transfection of non-viral DNA nanoparticles.Journal of peptide science : an official publication of the European Peptide Society · 2022Article
- Drug delivery to the central nervous system.Nature reviews. Materials · 2022Article
- Peptide-Assisted Nucleic Acid Delivery Systems on the Rise.International journal of molecular sciences · 2021Review
- In vitro and in vivo delivery of siRNA via VIPER polymer system to lung cells.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- Non-viral gene delivery systems for tissue repair and regeneration.Journal of translational medicine · 2018Review
- From Composition to Cure: A Systems Engineering Approach to Anticancer Drug Carriers.Angewandte Chemie (International ed. in English) · 2017Review
- Virus-Inspired Polymer for Efficient In Vitro and In Vivo Gene Delivery.Angewandte Chemie (International ed. in English) · 2016Article
- Non-Viral Nucleic Acid Delivery Strategies to the Central Nervous System.Frontiers in molecular neuroscience · 2016Review
- Journey to the Center of the Cell: Current Nanocarrier Design Strategies Targeting Biopharmaceuticals to the Cytoplasm and Nucleus.Current pharmaceutical design · 2016Review
- Multivalent display of pendant pro-apoptotic peptides increases cytotoxic activity.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- Reducible, dibromomaleimide-linked polymers for gene delivery.Biomaterials science · 2015Article
- Enhanced Performance of Plasmid DNA Polyplexes Stabilized by a Combination of Core Hydrophobicity and Surface PEGylation.Journal of materials chemistry. B · 2014Article
- Drug delivery systems, CNS protection, and the blood brain barrier.BioMed research international · 2014Review
- Maximizing gene delivery efficiencies of cationic helical polypeptides via balanced membrane penetration and cellular targeting.Biomaterials · 2014Article
- Optimization of Tet1 ligand density in HPMA-co-oligolysine copolymers for targeted neuronal gene delivery.Biomaterials · 2013Article
- Engineering biodegradable and multifunctional peptide-based polymers for gene delivery.Journal of biological engineering · 2013Article
- Block copolymers containing a hydrophobic domain of membrane-lytic peptides form micellar structures and are effective gene delivery agents.ACS macro letters · 2013Article
- Comparative study of guanidine-based and lysine-based brush copolymers for plasmid delivery.Biomaterials science · 2013Article
- Influence of histidine incorporation on buffer capacity and gene transfection efficiency of HPMA-co-oligolysine brush polymers.Biomacromolecules · 2013Article
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10 authors at 2 institutions in 1 country.
Funding
Abstract
Non-viral gene delivery systems capable of transfecting cells in the brain are critical in realizing the potential impact of nucleic acid therapeutics for diseases of the central nervous system. In this study, the membrane-lytic peptide melittin was incorporated into block copolymers synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization. The first block, designed for melittin conjugation, was composed of N-(2-hydroxypropyl)methacrylamide (HPMA) and pyridyl disulfide methacrylamide (PDSMA) and the second block, designed for DNA binding, was composed of oligo-l-lysine (K10) and HPMA. Melittin modified with cysteine at the C-terminus was conjugated to the polymers through the pyridyl disulfide pendent groups via disulfide exchange. The resulting pHgMelbHK10 copolymers are more membrane-lytic than melittin-free control polymers, and efficiently condensed plasmid DNA into salt-stable particles (~100-200 nm). The melittin-modified polymers transfected both HeLa and neuron-like PC-12 cells more efficiently than melittin-free polymers although toxicity associated with the melittin peptide was observed. Optimized formulations containing the luciferase reporter gene were delivered to mouse brain by intraventricular brain injections. Melittin-containing polyplexes produced about 35-fold higher luciferase activity in the brain compared to polyplexes without melittin. Thus, the melittin-containing block copolymers described in this work are promising materials for gene delivery to the brain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.