ArticleCytotherapy2013
Human adipose stromal vascular cell delivery in a fibrin spray.
Article in Cytotherapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02375802 (A Pilot Study), which is not on this map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A Pilot Study: Evaluating the Safety and Feasibility of Using Autologous Adipose-Derived Stromal Cells From Stromal Vascular Fraction (SVF) on Adults With Pressure Ulcers or Diabetic Foot Ulcers
Who cites it
25 citing papers in PubMed, 62 citations in OpenAlex.
- Alginate as a cytocompatible carrier for mechanically isolated stromal vascular fraction: an in vitro proof-of-concept study.Scientific reports · 2026Article
- A Novel Sprayable Fibrinogen/Glycosaminoglycans/Collagen-Based Bioink for Skin Wound Healing Applied by a Handheld Dual-Head Airbrush.Advanced healthcare materials · 2026Article
- Mechanical Purification of Lipofilling: The Relationship Between Cell Yield, Cell Growth, and Fat Volume Maintenance.Aesthetic plastic surgery · 2024Article
- Mechanical and Enzymatic Digestion of Autologous Fat Grafting (A-FG): Fat Volume Maintenance and AD-SVFs Amount in Comparison.Aesthetic plastic surgery · 2023Article
- Effervescent Atomizer as Novel Cell Spray Technology to Decrease the Gas-to-Liquid Ratio.Pharmaceutics · 2022Article
- Autologous Adipose-Derived Tissue Stromal Vascular Fraction (AD-tSVF) for Knee Osteoarthritis.International journal of molecular sciences · 2022Review
- Administration Methods of Mesenchymal Stem Cells in the Treatment of Burn Wounds.European burn journal · 2022Review
- Advances in spray products for skin regeneration.Bioactive materials · 2022Review
- Advances in Fibrin-Based Materials in Wound Repair: A Review.Molecules (Basel, Switzerland) · 2022Review
- Three states of stromal cells-solid, liquid, and aerosol-and innovative delivery methods not previously reported.Archives of plastic surgery · 2021Article
- Inter-center comparison of good manufacturing practices-compliant stromal vascular fraction and proposal for release acceptance criteria: a review of 364 productions.Stem cell research & therapy · 2021Review
- Supercharged Mechanical Stromal-cell Transfer (MEST).Plastic and reconstructive surgery. Global open · 2021Article
- Potential Using of Fat-derived Stromal Cells in the Treatment of Active Disease, and also, in Both Pre- and Post-Periods in COVID-19.Aging and disease · 2020Article
- Evaluation of Porcine Versus Human Mesenchymal Stromal Cells From Three Distinct Donor Locations for Cytotherapy.Frontiers in immunology · 2020Article
- Auto Micro Atomization Delivery of Human Epidermal Organoids Improves Therapeutic Effects for Skin Wound Healing.Frontiers in bioengineering and biotechnology · 2020Article
- Impact of the Different Preparation Methods to Obtain Human Adipose-Derived Stromal Vascular Fraction Cells (AD-SVFs) and Human Adipose-Derived Mesenchymal Stem Cells (AD-MSCs): Enzymatic Digestion Versus Mechanical Centrifugation.International journal of molecular sciences · 2019Review
- Fibrin Glue Enhances Adipose-Derived Stromal Cell Cytokine Secretion and Survival Conferring Accelerated Diabetic Wound Healing.Stem cells international · 2018Article
- In vivo evaluation of adipose- and muscle-derived stem cells as a treatment for nonhealing diabetic wounds using multimodal microscopy.Journal of biomedical optics · 2016Article
- Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs.Tissue engineering. Part C, Methods · 2016Article
- Challenges and Opportunities in Drug Delivery for Wound Healing.Advances in wound care · 2016Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
BACKGROUND
aimsAdipose tissue represents a practical source of autologous mesenchymal stromal cells (MSCs) and vascular-endothelial progenitor cells, available for regenerative therapy without in vitro expansion. One of the problems confronting the therapeutic application of such cells is how to immobilize them at the wound site. We evaluated in vitro the growth and differentiation of human adipose stromal vascular fraction (SVF) cells after delivery through the use of a fibrin spray system.
methodsSVF cells were harvested from four human adult patients undergoing elective abdominoplasty, through the use of the LipiVage system. After collagenase digestion, mesenchymal and endothelial progenitor cells (pericytes, supra-adventitial stromal cells, endothelial progenitors) were quantified by flow cytometry before culture. SVF cells were applied to culture vessels by means of the Tisseel fibrin spray system. SVF cell growth and differentiation were documented by immunofluorescence staining and photomicrography.
resultsSVF cells remained viable after application and were expanded up to 3 weeks, when they reached confluence and adipogenic differentiation. Under angiogenic conditions, SVF cells formed endothelial (vWF+, CD31+ and CD34+) tubules surrounded by CD146+ and α-smooth muscle actin+ perivascular/stromal cells.
conclusionsHuman adipose tissue is a rich source of autologous stem cells, which are readily available for regenerative applications such as wound healing, without in vitro expansion. Our results indicate that mesenchymal and endothelial progenitor cells, prepared in a closed system from unpassaged lipoaspirate samples, retain their growth and differentiation capacity when applied and immobilized on a substrate using a clinically approved fibrin sealant spray system.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.