ArticleBiophysical journal2012
Single-molecule observation of the induction of k-turn RNA structure on binding L7Ae protein.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Structure and ion-dependent folding of k-junctions.RNA (New York, N.Y.) · 2023Article
- The role of RNA structure in translational regulation by L7Ae protein in archaea.RNA (New York, N.Y.) · 2019Article
- Sequence determinants of the folding properties of box C/D kink-turns in RNA.RNA (New York, N.Y.) · 2017Article
- Control of box C/D snoRNP assembly by NEMBO reports · 2017Article
- Article
- A critical base pair in k-turns determines the conformational class adopted, and correlates with biological function.Nucleic acids research · 2016Article
- The solution structural ensembles of RNA kink-turn motifs and their protein complexes.Nature chemical biology · 2016Article
- The Kink Turn, a Key Architectural Element in RNA Structure.Journal of molecular biology · 2016Review
- Assembly and dynamics of the U4/U6 di-snRNP by single-molecule FRET.Nucleic acids research · 2015Article
- A critical base pair in k-turns that confers folding characteristics and correlates with biological function.Nature communications · 2014Article
- The K-turn motif in riboswitches and other RNA species.Biochimica et biophysica acta · 2014Review
- The k-junction motif in RNA structure.Nucleic acids research · 2014Article
- Structure of a rare non-standard sequence k-turn bound by L7Ae protein.Nucleic acids research · 2014Article
- Single-molecule characterization of Fen1 and Fen1/PCNA complexes acting on flap substrates.Nucleic acids research · 2014Article
- The molecular recognition of kink-turn structure by the L7Ae class of proteins.RNA (New York, N.Y.) · 2013Article
- The functional exchangeability of pk- and k-turns in RNA structure.RNA biology · 2013Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The k-turn is a commonly occurring structural motif that introduces a tight kink into duplex RNA. In free solution, it can exist in an extended form, or by folding into the kinked structure. Binding of proteins including the L7Ae family can induce the formation of the kinked geometry, raising the question of whether this occurs by passive selection of the kinked structure, or a more active process in which the protein manipulates the RNA structure. We have devised a single-molecule experiment whereby immobilized L7Ae protein binds Cy3-Cy5-labeled RNA from free solution. We find that all bound RNA is in the kinked geometry, with no evidence for transitions to an extended form at the millisecond timescale of the camera. Furthermore, real-time binding experiments provide no evidence for a more extended intermediate even at the earliest times, at a time resolution of 16 ms. The data support a passive conformational selection model by which the protein selects a fraction of RNA that is already in the kinked conformation, thereby drawing the equilibrium into this form.
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Registered trials
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